MAGE-3多抗原肽诱导抗非小细胞肺癌免疫效应研究  

Multiple antigen peptides derived from MAGE-3 elicit potent immune response against non-small cell lung cancer

作　　者：谢利海 何德全 

机构地区：[1]重庆万盛区南桐总医院呼吸科,重庆万盛400802

出　　处：《现代医药卫生》2010年第22期3390-3392,共3页Journal of Modern Medicine & Health

摘　　要：目的:探讨肿瘤抗原MAGE-3的多抗原表位肽能否有效诱导,对非小细胞肺癌产生免疫杀伤效应。方法:采用固相合成法合成多抗原肽(MAP),并经RP-HPLC纯化分析,质谱分析其分子量。诱导成熟树突状细胞负载MAGE-3MAP刺激淋巴细胞,采用标准51Cr释放实验检测特异性杀伤效率。结果:合成的MAGE-3MAP纯度大于95%,分子量测定值与理论值相符。MAP诱导的CTL对非小细胞肺癌的癌细胞杀伤效率高于MAGE-3单肽诱导的CTL,P<0.05。结论:MAGE-3来源的MAP能克服单表位肽抗原性弱的缺点,诱导较单肽更强烈的免疫反应。Objective：To study if multiple antigen peptides （MAPs） derived from MAGE-3 can elicit stronger immune response against non-small cell lung cancer than single strand epitope from MAGE-3.Methods：The peptides were synthesized with solid phase strategies,purified with reverse-phase HPLC and identified with mass spectrometry.We used mature dendritic cells loading peptides to stimulate CTL response.The immunogenicity and immune reactivity of MAP were evaluated on the basis of standard Cr release assay.51 Results：The CTLs elicited by MAPs could lyse non-small cell lung cancer cells more effectively （P0.05）.Conclusion：MAP derived from MAGE-3 can elicit stronger immune response against non-small cell lung cancer.

关 键 词：非小细胞肺癌 表位 肿瘤免疫治疗 

分 类 号：R73[医药卫生—肿瘤]