Survivin siRNA诱导人腺样囊性癌SACC-83细胞凋亡的实验研究  

Experiment Research about the Adenoid Cystic Carcinoma SACC-83 Cell Apoptosis Which Was Induced by Survivin siRNA

作　　者：饶国洲[1] 王华[2] 苍金荣[2] 李昂[1] 朱永进[1] 孙慧玲[1] 

机构地区：[1]西安交通大学医学院附属口腔医院中心实验室,西安710004 [2]陕西省人民医院,西安710068

出　　处：《现代检验医学杂志》2010年第5期51-53,共3页Journal of Modern Laboratory Medicine

摘　　要：目的 应用RNA干扰技术靶向阻断Survivin基因表达,探讨其对人腺样囊性癌SACC-83细胞的诱导凋亡作用.方法 实验设空白对照组,RNA干扰阴性对照组和RNA干扰组.将RNA干扰载体通过Lipofection介导转染腺样囊性癌细胞.采用倒置显微镜观察细胞凋亡形态;流式细胞仪检测细胞凋亡峰;透射电镜观察细胞凋亡超微结构.结果 转染细胞48 h后RNA干扰组细胞形态学发生改变,呈典型的细胞凋亡形态.流式细胞仪检测在细胞周期G1/G0期前出现亚二倍体凋亡峰,电镜超微结构可见凋亡小体.空白对照组,RNA干扰阴性对照组无此变化.结论 Survivin siRNA干扰能诱导体外培养的人腺样囊性癌SACC-83细胞发生凋亡,以Survivin 为靶点有望成为肿瘤基因治疗的可行性.Objective To investigated the impact of human adenoid cystic carcinoma SACC-83 cell apoptosis induced by the application of RNA interference targeting Survivin gene expression block. Metheds The experiment consisted of the blank control group,RNA interference,RNA interference negative control group. The RNA interference vector mediated trans- fection adenoid cystic carcinoma by Lipofection. The cell apoptosis morphology was observed under the inverted microscope. Apoptosis was detected by flow cytometry peak was detected by flow cytometry. The cell apoptosis ultrastructure was detected by electron microscopy. Results The cell morphology of RNA interference changed into a typical morpholo- gy of apoptosis after 48h of transfected cells. Flow cytometry explored the hypodiploid apoptotic peak in cell cycle G1/G0 phase before,and apoptotic bodies under electron microscopy. Control group and RNA interference negative control group had no such change. Conclusion Survivin siRNA interference could induce cultured human adenoid cystic carcinoma SACC-83 cell apoptosis. If survivin targeting is expected,it will become the feasibility of cancer gene therapy.

关 键 词：RNA干扰 细胞凋亡 流式细胞术 腺样囊性癌细胞 

分 类 号：R730.43[医药卫生—肿瘤]