玻璃体切割术后再形成液和晶状体中TGF-β2含量的变化  

Changes of transforming growth factor-β2 levels in intravitreous regenerated fluid and lens extracts after vitrectomy

作　　者：王丹[1] 严宏[1] 刘晓萃[2] 王萍[1] 

机构地区：[1]第四军医大学唐都医院眼科,陕西省西安市710038 [2]解放军三零一医院眼科,北京市100852

出　　处：《眼科新进展》2010年第10期905-908,共4页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:30872837)~~

摘　　要：目的测定行兔眼玻璃体切割术后玻璃体再形成液及晶状体中的转化生长因子β2（transforming growth factor-β2,TGF-β2）的含量,探讨其的变化在玻璃体切割术后白内障形成中的作用。方法 6月龄雄性新西兰大白兔24只,体质量2.3~2.4kg,随机分为3组,每组8只,其中A组左眼行单纯玻璃体次全切除术,B组左眼行玻璃体次全切除术＋0.5mL13.2g.L-1N-乙酰半胱氨酸（N-acetylcysteine,NAC）玻璃体内注射,C组左眼行玻璃体次全切除术＋0.5mL65.2g.L-1NAC玻璃体内注射;A组右眼不做任何处理作为对照组。术后5个月过量麻醉法处死各组兔,其间观察晶状体形态变化,取各组兔眼球收集玻璃体再形成液及晶状体,观察晶状体纤维细胞形态学变化,并使用兔TGF-β2ELISA试剂盒检测再形成液及晶状体水溶性蛋白质中TGF-β2的含量。结果术后5个月,各组晶状体均未出现明显的混浊,晶状体纤维细胞无明显异常。A、B、C组玻璃体再形成液中TGF-β2含量与对照组玻璃体液中TGF-β2含量相比均有明显的增加,分别增加了125.30%、80.40%和88.93%（均为P〈0.05）;而A、B、C组之间相比,差异均无统计学意义（均为P〉0.05）。A、B、C组晶状体水溶性蛋白质中TGF-β2的含量与对照组相比也有了明显的增加,分别增加了112.40%、102.09%和81.18%（均为P〈0.05）;而A、B、C组之间相比,差异均无统计学意义（均为P〉0.05）。结论玻璃体切割术可引起玻璃体再形成液和晶状体中TGF-β2含量的增加,TGF-β2含量的增加可能与玻璃体切割术后白内障的形成有关。Objective To investigate the concentration of transforming growth factor-β2（TGF-β2）in intravitreous regenerated fluid and lens extracts after successful vitrectomy,and evaluate its importance on the cataract formation after vitrectomy.Methods Twenty four six-month male New Zealand albino rabbits were randomly divided into 3 groups：group A,group B and group C,8 rabbits in each group.Left eyes in group A were treated with simple vitreous subtotal ectomy;Left eyes in group B were injected with 0.5 mL 13.2 g·L-1 N-acetylcysteine into the vitreous cavity immediately and vitreous subtotal ectomy;Left eyes in group C were injected with 0.5 mL 65.2 g·L-1 N-acetylcysteine into the vitreous cavity immediately and vitreous subtotal ectomy.And there was no treatment in the right eyes of group A as control group.These rabbits were sacrificed by overdose anesthesia at postopertive 5 months.The transparence of the lenses and the quantity of TGF-β2 in the vitreous regenerated fluid and the water-soluble protein of lens extracts were measured by TGF-β2 ELISA kit.Results There was no significant morphological change or opacification of lens detected at postoperative 5 months.However,levels of TGF-β2 in the vitreous regenerated fluid of group A,group B and group C increased 125.30%,80.40% and 88.93% compared with that in control group（all P0.05）,while no difference was found between each two of these three groups（both P0.05）.The levels of TGF-β2 in water-soluble protein of lens extracts in group A,group B and group C were 112.40%,102.09% and 81.18% higher than that in control group（all P0.05）,while no difference was found between each two of these 3 groups（both P0.05）.Conclusion Vitrectomy can increase TGF-β2 levels in the regenerated fluid of vitreous and water-soluble protein of lens extracts,which have relation with cataract after vitrectomy.

关 键 词：玻璃体切割术 转化生长因子-Β2 玻璃体 晶状体 兔 

分 类 号：R776[医药卫生—眼科] Q959.836[医药卫生—临床医学]