大鼠角膜碱、酸、热烧伤后ICAM-1、VCAM-1、CD44及E-selectin mRNA的表达  

Expression of ICAM-1,VCAM-1,CD44 and E-selectin mRNA after corneal alkali burn,acid burn and thermal burn in rat corneas

作　　者：李雪[1] 徐锦堂[2] 崔浩[1] 胡琦[1] 

机构地区：[1]哈尔滨医科大学第一附属医院眼科视光学中心,黑龙江省哈尔滨市150001 [2]暨南大学医学院第一附属医院眼科,广东省广州市510632

出　　处：《眼科新进展》2010年第10期928-932,共5页Recent Advances in Ophthalmology

基　　金：黑龙江省教育厅科学技术研究项目(编号:11521163)~~

摘　　要：目的利用RT-PCR法检测大鼠角膜烧伤后角膜局部ICAM-1、VCAM-1、CD44及E-selectin的mRNA表达情况,探讨黏附分子在角膜烧伤中的变化,以进一步完善角膜烧伤的免疫机制。方法制备Wistar大鼠角膜碱、酸、热烧伤模型。提取大鼠烧伤后3d和2周角膜RNA,反转录扩增ICAM-1、VCAM-1、CD44及E-selectin,根据DNA条带的光密度值确定DNA含量。观察烧伤后3d和2周以上各指标的变化。结果角膜烧伤后3d,与正常对照组(1.658±0.722)相比,3个烧伤组ICAM-1mR-NA表达量增加(5.699±0.934、3.182±0.830、3.403±0.916),差异均有显著统计学意义(均为P<0.01);2周时,3个烧伤组ICAM-1mRNA较3d时明显减少,其中碱烧伤组伤后3d和2周ICAM-1mRNA的表达量均较酸和热烧伤组明显增多,差异均有显著统计学意义(均为P<0.01)。同正常对照组(2.172±0.368)相比,烧伤后2周时,碱烧伤组VCAM-1mRNA表达量(8.591±1.021)增多,差异有极显著统计学意义(P<0.001),而酸和热烧伤组差异均无显著统计学意义。同正常对照组(1.402±0.439)相比,伤后3d时3个烧伤组CD44mRNA表达量无显著变化,2周时碱烧伤组CD44mRNA表达量(5.587±0.753)增加,与对照组比较,差异有显著统计学意义(P<0.01),酸和热烧伤组CD44mRNA表达量增加,但差异无统计学意义。3d时角膜各烧伤组E-selectin的表达量低于正常对照组,差异均有显著统计学意义(均为P<0.01);2周时各烧伤组E-selectinmRNA的表达量明显增加,高于正常对照组,差异均有显著统计学意义(均为P<0.01),3个烧伤组间差异无显著统计学意义。结论在角膜烧伤的急性期,ICAM-1介导中性粒细胞与血管内皮细胞的黏附,表达增加。CD44及其配体在角膜烧伤特别是角膜碱烧伤的愈合过程中发挥重要作用。作为介导白细胞与血管内皮细胞最初黏附过程的黏附分子,E-selectin在角膜烧伤的病理发生中的作用机制有待于进一步研究。Objective To investigate the changes of adhesion molecules in corneal burn to complete the immunologic mechanisms of corneal burn by detecting the expression of ICAM-1,VCAM-1,CD44 and E-selectin mRNA after alkali burn,acid burn and thermal burn in the rat corneas by RT-PCR.Methods Wistar rat models of alkali burn,acid burn and thermal burn were made.Corneal RNA was extracted at 3 days and 2 weeks after corneal burn.ICAM-1,VCAM-1,CD44 and E-selectin were amplified with reverse transcription.DNA content was determined by optical density of DNA strap.Changes of each index were observed at 3 days and 2 weeks after corneal burn.Results The expression of ICAM-1 mRNA were 5.699±0.934,3.182±0.830 and 3.403±0.916 at 3 days after corneal alkali burn,acid burn and thermal burn higher than 1.658±0.722 in normal control group,there were significant differences（all P0.01）.The expression of ICAM-1 mRNA at 2 weeks after corneal burn was fewer than those at 3 days after corneal burn（P0.01）.At 3 days and 2 weeks after corneal burn,expression of ICAM-1 mRNA in alkali burn group was obviously higher than those in acid burn group and thermal burn group after corneal burn,there were significant differences（all P0.01）.At 2 weeks after corneal burn,the expression of VCAM-1 mRNA was 8.591±1.021 in alkali burn group higher than 2.172±0.368 in normal control group,and there was significant difference（P0.001）.While no obvious difference was found in expression of VCAM-1 mRNA for acid burn group and thermal group compared with normal control group.At 3 days after corneal burn,there was no obvious difference in expression of CD44 mRNA of 3 corneal burn groups compared with 1.402±0.439 in normal control group.At 2 weeks after corneal burn,expression of CD44 mRNA was 5.587±0.753 in alkali burn group higher than that in normal control group,there was significant difference（P0.01）;Expression of CD44 mRNA increased at 2 weeks after corneal burn in acid burn group and thermal burn group,but no difference was found.At 3 d

关 键 词：角膜 碱烧伤 酸烧伤 热烧伤 ICAM-1 VCAM-1 CD44 E-SELECTIN 

分 类 号：R777.2[医药卫生—眼科]