瘦素和血管内皮生长因子在碱烧伤诱导的大鼠角膜新生血管模型中的表达  

Expression of leptin and vascular endothelial growth factor in alkali burn induced corneal neovascularization in rat models

作　　者：潘婕[1] 张明昌[2] 张洁[2] 孙广莉[2] 

机构地区：[1]荆门市第一人民医院,湖北省荆门市448000 [2]华中科技大学同济医学院附属协和医院眼科,湖北省武汉市430030

出　　处：《眼科新进展》2010年第10期941-944,共4页Recent Advances in Ophthalmology

摘　　要：目的观察碱烧伤后不同时期角膜的组织病理学变化,以及瘦素和血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达,探讨它们与角膜新生血管(corneal neovascularization,CNV)的关系。方法使用碱烧伤诱导大鼠角膜新生血管模型,采用浸润1mol.L-1NaOH溶液、直径为3mm的单片滤纸,准确置于大鼠右眼角膜中央30s,制作CNV模型。25只SD大鼠右眼为实验眼,随机分成5组,每组5眼,左眼为正常对照眼。每天用裂隙灯显微镜观察角膜新生血管,分别计算新生血管长度和面积,并行HE染色和免疫组织化学检测。结果碱烧伤后4d、7d、14d、21d,实验组CNV面积分别为:(2.55±0.15)mm2、(4.15±0.36)mm2、(10.21±0.45)mm2、(8.56±0.06)mm2。免疫组织化学检测显示角膜碱烧伤后1d,实验组瘦素、VEGF的表达开始增高,碱烧伤后14d达高峰,14d后瘦素、VEGF的表达下降,21d后显著下降;其表达部位主要分布在形成的新生血管区域和上皮层;正常对照组瘦素可微弱表达于角膜上皮层和基质层,VEGF没有表达或仅在上皮基底膜有微弱表达。实验组角膜瘦素、VEGF阳性表达率较对照组明显增加(χ2=29.07,P=0.001;χ2=35.51,P=0.001),瘦素与VEGF的表达具有正相关性(r=0.95,P=0.001)。结论瘦素和VEGF表达水平与角膜新生血管的生成具有相关性,瘦素可能成为治疗CNV的靶点。Objective To investigate the correlation of leptin and vascular endothelial growth factor（VEGF）with corneal neovascularization（CNV）by observing histopathological changes of corneas at different stages,expression of leptin and VEGF after alkali burn.Methods CNV models were established by putting the filter paper with the diameter of 3 mm immersed into 1 mol·L-1 NaOH solution on the central corneas of right eyes of rats for 30 seconds.Right eyes of 25 SD rats were treated as experimental group,and divided into 5 groups,5 eyes in each group.Left eyes were treated as normal control group.Slit lamp microscope was used to observe CNV every day;Length and area of CNV were calculated and detected by HE staining and immunohistochemical method.Results CNV areas were（2.55±0.15）mm2,（4.15±0.36）mm2,（10.21±0.45）mm2 and（8.56±0.06）mm2 at the 4th day,the 7th day,the 14th day and the 21st day.Immunohistochemical method showed leptin and VEGF started to express at 1 day after alkali burn,went to peak at 14 days,decreased 14 days later and obviously decreased at 21 days after alkali burn.In normal control group,leptin slightly expressed in corneal epithelial layer and stromal layer,VEGF had no expression or only slightly expressed in basement membrane of epithelium.In experimental group,the positive expression of leptin and VEGF were obviously higher than those in normal control group（χ2=29.07,P=0.001;χ2=35.51,P=0.001）,and they mainly expressed in neovascular area and epithelial layer.There was positive correlation between expression of leptin and VEGF（r=0.95,P=0.001）.Conclusion Expression levels of leptin and VEGF have relation with CNV.Leptin can become the target to treat CNV.

关 键 词：碱烧伤 角膜新生血管 瘦素 血管内皮生长因子 

分 类 号：R772.2[医药卫生—眼科]