CD4^+CD25^+调节性T细胞在自身免疫性肝炎发病中的作用  

Role of CD4^+CD25^+ regulatory T cells on the pathogenesis of autoimmune hepatitis

作　　者：周慧[1] 朱丽影[2] 王威[3] 颜炳柱[3] 陈立艳[3] 

机构地区：[1]哈尔滨医科大学附属第一医院感染科,黑龙江哈尔滨150001 [2]哈尔滨医科大学附属第四医院感染科,黑龙江哈尔滨150001 [3]哈尔滨医科大学附属第二医院感染科,黑龙江哈尔滨150086

出　　处：《现代生物医学进展》2010年第18期3447-3450,共4页Progress in Modern Biomedicine

基　　金：黑龙江省卫生厅基金资助(2007-278)

摘　　要：目的:比较自身免疫性肝炎(autoimmune hepatitis,AIH)患者与健康对照者(healthy controls,HCs)外周血CD4+CD25+调节性T细胞(CD4+CD25+Tregs)数量、免疫抑制功能的变化,探讨CD4+CD25+Tregs参与AIH发病的可能机制。方法:采用流式细胞仪检测8例AIH患者及15例健康对照组的外周血CD4+CD25+Tregs数量的百分比及绝对数量;采用共同培养方法检测AIH患者外周血CD4+CD25+Tregs的免疫抑制功能的变化;实时荧光定量聚合酶链反应(RT-FQ-PCR)检测AIH患者外周血CD4+CD25+Tregs中FoxP3mRNA的表达。结果:AIH患者外周血CD4+CD25+Tregs数量明显低于HCs(p<0.01);混合淋巴细胞共同培养结果显示,AIH患者外周血CD4+CD25+Tregs抑制功能明显低于HCs组(p<0.01);AIH患者外周血CD4+CD25+Tregs的FoxP3mRNA相对表达量显著降低,与HCs组比较有显著性差异(p<0.01)。结论:CD4+CD25+Tregs细胞的数量的减少和Foxp3表达的降低所造成的CD4+CD25+Tregs细胞免疫抑制功能受损可能是AIH发病的一个因素。Objective：To analyze the number,suppressive function of CD4 ＋ CD25 ＋ regulatory T cells（CD4 ＋ CD25 ＋ Tregs）in pe-ripheral blood from patients with autoimmune hepatitis（AIH）and healthy controls（HCs）and research the possible mechanisms that CD4 ＋ CD25 ＋ Tregs participate in the onset of AIH.Methods：Flow cytometry was used to analyze the frequency of CD4 ＋ CD25 ＋ Tregs from 8 patients with AIH and 15 healthy controls;Functional characterization of CD4 ＋ CD25 ＋ Tregs from the peripheral blood mononu-clear cells（PBMC）of patients and HCs were analyzed by suppression of proliferation by co-cultured effector CD4 ＋ CD25 ＋ T cells.Foxp3 message（mRNA）expression level was assessed by quantitative real-time polymerase chain reaction.Results：The proportion of CD4 ＋ CD25 ＋ Tregs from the patients with AIH was significantly decreased as compared with the healthy controls（P0.01）;CD4 ＋ CD25 ＋ Tregs from patients with AIH exhibited hampered inhibition of responder CD4 ＋ CD25-cell proliferation compared with those from healthy controls（P0.01）;CD4 ＋ CD25 ＋ Tregs from patients with AIH expressed lower levels of FoxP3 mRNA.Conclusions：Decreased amount of CD4 ＋ CD25 ＋ Tregs and decreased expression of Foxp3 impair the suppressive activity of CD4 ＋ CD25 ＋ Tregs,which may be a factor in the pathogenesis of AIH.

关 键 词：自身免疫性肝炎(AIH) CD4+CD25+调节性T细胞 FOXP3 

分 类 号：R575.1[医药卫生—消化系统]