海洛因对神经元原癌基因c-fos表达的抑制作用  

The Suppression of c-fos in Neuron Apoptosis Induced by Heroin

作　　者：卢佩彦[1] 李泽鸿[1] 郭勇[1] 竞花兰[1] 林文盈 刘小山[1] 

机构地区：[1]中山大学中山医学院法医学系,广东广州510080 [2]阳江市公安局刑事科学技术研究所,广东阳江529500

出　　处：《法医学杂志》2010年第5期321-324,共4页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(30860318)

摘　　要：目的观察海洛因对SD乳鼠小脑颗粒神经元c-fos蛋白表达的影响,探讨海洛因对神经元的损伤机制。方法体外培养原代小脑颗粒神经元并建立海洛因促神经元凋亡模型,用逆转录聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)和Western印迹法技术,检测小脑颗粒神经元凋亡时c-fos的表达变化。结果海洛因质量浓度为10μg/mL时小脑颗粒神经元于48h即出现明显凋亡,RT-PCR和Western印迹法均提示原癌基因c-fos在神经元凋亡时表达明显下调。结论海洛因通过抑制原癌基因c-fos的表达来诱导小脑颗粒神经元的凋亡,提示c-fos的表达下调可能是海洛因促使神经元凋亡的机制之一。Objective To explore the changes of c-fos in apoptosis of cerebellar granular neuron of neonatal SD rats induced by heroin and the mechanisms of neuronal injury caused by heroin.Methods Primary cerebellar granular neuron were cultured in vitro,the model of apoptosis induced by heroin was established.Reverse transcription-polymerase chain reaction（RT-PCR） and Western blotting were adopted to investigate the changes of c-fos in cell models.Results Ten μg/mL of heroin was the optimal dose to induce the apoptosis of cerebellar granular neuron at 48 h.Both Western blotting and RT-PCR showed down regulation of c-fos expression.Conclusion Heroin could induce apoptosis of cerebellar granular neuron and down regulation of c-fos,which may be one of the apoptosis mechanisms.

关 键 词：法医病理学 海洛因 神经元 基因 fos 大鼠 

分 类 号：R736.1[医药卫生—肿瘤]