免疫组织化学法检测HO-1在人挫伤脑组织中的表达  

Immunohistochemical Expression of HO-1 in Traumatic Human Brain Tissue

作　　者：彭雪[1] 靳占峰[1] 邵洪江[1] 高铁磊[1] 李忠[2] 张颖慧 

机构地区：[1]哈尔滨医科大学法医学教研室,黑龙江哈尔滨150081 [2]哈尔滨医科大学附属第一医院,黑龙江哈尔滨150081 [3]哈尔滨市公安局刑事技术支队,黑龙江哈尔滨150026

出　　处：《法医学杂志》2010年第5期325-327,331,共4页Journal of Forensic Medicine

基　　金：黑龙江省政府博士后科研启动基金(LRB07-144)

摘　　要：目的研究人脑挫伤后不同时间血红素加氧酶-1(heme oxygenase-1,HO-1)表达的变化,为法医学脑损伤时间的推断提供依据。方法选择24例因颅脑损伤死亡案例为损伤组,4例非颅脑损伤死亡的案例为对照组,应用免疫组织化学和图像分析技术,分别于脑损伤后存活时间3h、6～9h、12～24h、36h～3d、5～8d及17～20d检测脑组织标本中HO-1随时间的变化规律。结果经统计学分析,HO-1阳性细胞表达在伤后3h以内组开始升高,12～24h组表达强度最高,36h～3d组仍见高表达,以后逐渐下降。各组组间及与对照组相比差异有统计学意义(P<0.05)。结论 HO-1的表达可能成为实际检案中脑损伤时间推断的诊断指标。Objective To investigate the expression of heme oxygenase-1（HO-1） at different intervals and to provide evidence for estimation on injury intervals after brain contusion in human.Methods Twenty-four patients died of serious brain injury were assigned as injury group and 4 patients died of non-brain injury were served as control group.HO-1 expression was analyzed in brain tissue at different time intervals（3 h,6-9 h,12-24 h,36 h-3 d,5-8 d,17-20 d） by immunohistochemistry and auto-image analysis system.Results The level of HO-1 expression started to increase in 3 h after brain contusion compared to the control group（P0.05）.The level of HO-1 expression highest level in 12-24 h group,and maintained high level in 36 h-3 d,then decreased gradually.Conclusion The expression of HO-1 might be a strong evidence for human brain contusion time estimation.

关 键 词：法医病理学 脑损伤 血红素加氧酶-1 免疫组织化学 损伤时间 

分 类 号：R735.2[医药卫生—肿瘤]