PPARγ配体扎鲁司特对氧化偶氮甲烷诱导的大鼠大肠癌的预防作用  

Preventive effects of Zafirlukast on colorectal cancer induced by azoxymethane in rats

作　　者：谭志洁[1] 梁俊英[1] 崔健[1] 

机构地区：[1]山东省济南市市中区人民医院内科,250002

出　　处：《中国临床研究》2010年第10期847-849,共3页Chinese Journal of Clinical Research

摘　　要：目的探讨过氧化物酶体增殖物激活受体γ(PPARγ)的合成配体扎鲁司特,对氧化偶氮甲烷(AOM)诱导的大鼠大肠畸变隐窝病灶(ACF)形成及大肠癌的化学预防作用。方法利用AOM诱导大鼠大肠癌模型,实验大鼠随机分为3组:对照组、AOM组(AOM诱癌)、扎鲁司特预防组(AOM诱癌+0.15%扎鲁司特)。应用美蓝染色法观察各组第12周大鼠大肠ACF、大肠畸变隐窝(AC)的数量以及计算各组第32周时大鼠大肠癌的发生率,并加以比较。结果实验第12周时除对照组外,AOM组及扎鲁司特预防组均有ACF形成,扎鲁司特预防组大肠ACF和AC的平均数明显低于AOM组(P均<0.05);实验第32周时除对照组外,AOM组、扎鲁司特预防组大鼠均检出大肠癌,大肠癌的发生率分别为72.22%(13/18)、42.11%(8/19),扎鲁司特预防组大肠癌的发生率明显低于AOM组(P<0.05)。结论扎鲁司特能抑制大鼠诱癌早期ACF的数量,对AOM诱导的大鼠大肠癌有明显的化学预防作用。Objective Zafirlukast is a peroxisome proliferator-activated receptor gamma（PPARγ） ligand. The aim in this study is to explore the ehemopreventive effects of Zafirlukast on the formation of aberrant crypt loci （ACF） of rat＇s large intestine and colorectal cancer induced by azoxymethane （ AOM ）. Methods Colorectal cancer of rat was induced by AOM intraperitoneal injection. After adaptive feed for I week,80 Wistar rats were randomly divided into three groups： control group （ ordinary diet feed, n = 20） , AOM group （ ordinary diet ＋ AOM inducing cancer, n = 30 ） and prevention group （ Zafirlukast feed plus AOM inducing cancer,n =30）. The numbers of ACF and aberrant crypt （AC） in large intestine were observed by methylene blue staining at 12th week of experiment, and the incidence of colorectal cancer were calculated and compared at the end of experiment. Results At 12th week of experiment, the ACF were found in AOM group and prevention group, and the average number of ACF and AC in prevention group were lower than those in AOM group （all P 〈 0. 05 ） ;In control group, the ACF were not found. At 32th week of experiment, the colorectal cancers were detected in AOM group and prevention group. The incidence of colorectal cancer was 72. 22% （ 13/18 ） in AOM group against 42. 11% （8/19） in prevention group, and there was significant difference in incidence of colorectal cancer between prevention group and AOM group （ P 〈 0. 05 ）. Conclusions Zafirlukast can decrease the number of ACF and AC in the early stage of experiment and significantly prevent the production of colorectal cancer induced by AOM.

关 键 词：扎鲁司特 过氧化物酶体增殖物激活受体Γ 大肠癌 氧化偶氮甲烷 大鼠 

分 类 号：R735.3[医药卫生—肿瘤]