雄黄对多发性骨髓瘤细胞株RPMI8226细胞基因表达谱的作用  

Gene Expression Profile of Multiple Myeloma Cell Line Treated by Realgar

作　　者：王孟昌[1] 刘蓬勃[1] 

机构地区：[1]西安交通大学第一医院血液科,陕西西安710061

出　　处：《临床医学工程》2010年第10期1-3,共3页Clinical Medicine & Engineering

基　　金：国家自然基金项目(编号:81071952)

摘　　要：目的阐明雄黄治疗多发性骨髓瘤的分子机制。方法应用cDNA芯片在不同3天时间比较雄黄治疗前后72小时多发性骨髓瘤细胞系基因表达谱的改变。结果 cDNA芯片结果显示54条基因上调,60条基因下调,进一步分析发现17条上调基因Z-score大于2,3条下调基因Z-score小于-2。结论这些基因是本研究中雄黄治疗后显著改变的基因,CCL2,CCL3,BTG1,TNFAIP3,TNFAIP8,SLC38A2,IGFBP4是重要的上调基因,涉及一系列的细胞生命活动如:细胞增殖、细胞间信号转导、凋亡调节及细胞内环境稳定。有3个显著下调的基因涉及到肌肉收缩。部分基因(CCL2,TNFAIP3,BTG1)的结果通过反转录聚合酶链反应证实。Objective In order to elucidate the molecular mechanism of realgar treatment for multiple myeloma（MM） . Methods cDNA microarray was used to compare the gene expression profile of MM cell line RPMI8226 at 72 hrs pre-and post-realgar treatment on three separate days. Results 54 up-regulated and 60 down-regulated genes were identified by cDNA microarray,further analysis screened out 17 up-regulated and 3 down-regulated genes with Z-score greater than 2 or less than-2. Conclusion These genes can be considered the significantly altered genes after realgar treatment in this study. CCL2,CCL3,BTG1,TNFAIP3,TNFAIP8,SLC38A2,IGFBP4 are importment up-regulated genes and they are associated with a variety of cell life functions such as cell growth,cell-cell signaling,regulation of apoptosis and cell homeostasis based on biological process of gene. There are only 3 significantly down-regulated genes（Z-score -2.0） involved in muscle contract. Subsequent validation of selected genes（CCL2,TNFAIP3,BTG1） by reverse transcription polymerase chain reaction is consistent with our microarray analysis.

关 键 词：雄黄 基因芯片 多发性骨髓瘤 RPMI8226细胞 

分 类 号：R733.3[医药卫生—肿瘤]