苯酚降解菌CN-6的分离及其生长特性  

Biodegradation of Phenol by a Novel Isolated Bacterium Pseudomonas sp. CN-6

作　　者：龙腾锐[1] 张钊[1,2] 荘瑞鑫 

机构地区：[1]重庆大学三峡库区生态与环境教育部重点实验室,重庆400045 [2]台湾元智大学工程学院化学工程与材料科学系,中国台湾32003

出　　处：《土木建筑与环境工程》2010年第5期82-87,共6页Journal of Civil,Architectural & Environment Engineering

基　　金：台湾科学委员会基金资助项目(NSC96-2628-E-155-002-MY2)

摘　　要：从台湾某纺织厂污泥中分离得到一株能够将苯酚作为唯一碳源和能源生长的细菌。根据其形态和生理生化反应结合16S rDNA测定分析,该菌被鉴定为假单胞杆菌(Pseudomonas sp.)。降解特性研究表明,该菌降解苯酚的最适pH和温度分别为8.5和30℃,在此条件下,浓度高达500mg/L的苯酚能被该菌在17 h内将完全降解。在动力学研究中,由于高浓度苯酚对CN-6菌株的降解过程存在底物抑制现象,故采用Haldane非竞争性底物抑制模型,计算确定了模型参数μmax、KS和KI分别为0.452 h-12、8.617 mg/L7、82.4 mg/L,该动力学方程对实验数据能很好拟合。A strain that have capable of metabolizing phenol as a sole source of carbon and energy sources was isolated from an sludge collected from textile chemical plant in Taiwan. It was identified as Pseudomonas sp. based on its morphology, physiological, biochemical characteristics with an automatic biometrical system and the 16S rDNA sequence analysis. It is shown that the biodegradation of phenol was optimized at culture medium pH 8.5 and 30℃. Under the condition, the strain degraded phenol completely within 17h even though the initial phenol level was high to 500 mg/L. With the study of dynamic growth kinetics of suspended cells, the value of μmax , Ks and K1 were found to be 0. 452 h^-1 , 28. 617 rag/L, 782.4 mg/L for strain CN-6 degrading phenol using Haldane＇s inhibition kinetic models.

关 键 词：苯酚 生物降解 分离 动力学 PSEUDOMONAS sp. 

分 类 号：X172[环境科学与工程—环境科学]