高脂饮食及耐力训练对大鼠腓肠肌p53和IL-6表达的影响  

Effects of High-fat Diets and Endurance Training on p53 and IL-6 Expression of Gastrocnemius Muscle in Rats

作　　者：何强[1] 漆正堂[1] 丁树哲[1] 

机构地区：[1]华东师范大学体育与健康学院,上海200241

出　　处：《南京体育学院学报（自然科学版）》2010年第3期21-22,18,共3页Joournal of Nanjing Institute of Physical Education:Natural Science

基　　金：上海市浦江人才计划44038470;教育部新世纪人才支持计划790013P8

摘　　要：目的:探讨高脂饮食和耐力训练对大鼠腓肠肌p53和IL-6表达的影响。方法:28只大鼠随机分成4组:高脂对照组(H)、高脂耐力组(R)、普通对照组(C)和普通耐力组(E),每组7只,训练8周;末次训练后24h,各组大鼠在安静状态下处死,取腓肠肌。采用ELISA法测定腓肠肌胞浆中p53和IL-6的含量。结果:(1)E组大鼠肌浆中p53的表达显著低于C组(p<0.05),H组与C组比较无显著性差异,R组与E组比较也无显著性差异;(2)H组大鼠肌浆中IL-6的表达显著高于C组(p<0.05),R组也显著高于E组(p<0.05),但C组与E组比较无显著性差异。结论:高脂饮食不仅会引起大鼠在安静状态时的炎症反应,还会促进大鼠运动时出现的运动性机体改善;耐力训练对大鼠骨骼肌的衰老具有一定的延缓作用,IL-6对长时间的耐力运动具有一定的适应性。Aim：To investigate the effects of high-fat diets and endurance training on p53 and IL-6 in rats＇ gastrocnemius muscle. Methods：Twenty-eight male SD rats were randomly and equally divided into four groups：Control group with high-fat diets （H）,Endurance training group with high-fat diets （R）,Control group with ordinary diet （C）,Endurance training group with ordinary diet （E）. The rats were sampled after 8-week exercise training. 24h after the last training,the rats were killed ,gastrocnemius muscle were excised quickly for ELISA. Results：（1）The p53 expression of gastrocnemius muscle in E group was downregulated significantly,compaired with C（p0.05）,but there is no significant change in H and C,as well as R and E; （2） the IL-6 expression of gastrocnemius muscle in H group was upregulated significantly,compaired with C （p0.05）,that in R was also upregulated significantly,compaired with E（p0.05）.But there is no significant change in C and E. Conclusion：High-fat diets could not only lead to inflammatory reaction when rest,but also bring about a series of improvements to athletic body; endurance training could delay aging of the muscle,The expression of IL-6 would adape to the long-term endurance training.

关 键 词：P53 IL-6 大鼠 高脂饮食 耐力训练 

分 类 号：G804.7[文化科学—运动人体科学]