Aerobic biodegradation of diethyl phthalate by Acinetobacter sp. JDC-16 isolated from river sludge  

作　　者：梁任星 吴学玲 王兴娜 代沁芸 王洋洋 

机构地区：[1]School of Minerals Processing and Bioengineering,Central South University

出　　处：《Journal of Central South University》2010年第5期959-966,共8页中南大学学报（英文版）

基　　金：Project(30770388) supported by the National Natural Science Foundation of China

摘　　要：A gram negative bacterium,named JDC-16,which can grow well on the substrate of phthalic acid esters(PAEs) as the sole source of carbon and energy,was isolated from river sludge.Based on the morphology,physiological and biochemical properties and analysis of 16S rRNA gene sequence,it was preliminarily identified belonging to the genus Acinetobacter.The result of substrates utilization range indicates that strain JDC-16 can utilize a variety of phthalates except for diisononyl phthalate(DINP) .The degradation tests using diethyl phthalate(DEP) as the model compound show that the optimal pH and temperature for DEP degradation by Acinetobacter sp.JDC-16 is 8.0 and 35℃,respectively.Meanwhile,degradation kinetics under various initial concentrations of DEP reveals that substrate depletion curves fit well with the modified Gompertz model with high correlation coefficient(R 2 >0.99) .Furthermore,the substrate induction test indicates that DEP-induction can apparently shorten the lag phase and enhance the degradation rate.This work highlights the potential of this isolate for bioremediation of phthalates-contaminated environments.A gram negative bacterium, named JDC-16, which can grow well on the substrate of phthalic acid esters （PAEs） as the sole source of carbon and energy, was isolated from river sludge. Based on the morphology, physiological and biochemical properties and analysis of 16S rRNA gene sequence, it was preliminarily identified belonging to the genus Acinetobacter. The result of substrates utilization range indicates that strain JDC-16 can utilize a variety of phthalates except for diisononyl phthalate （DINP）. The degradation tests using diethyl phthalate （DEP） as the model compound show that the optimal pH and temperature for DEP degradation by Acinetobacter sp. JDC-16 is 8.0 and 35 ℃, respectively. Meanwhile, degradation kinetics under various initial concentrations of DEP reveals that substrate depletion curves fit well with the modified Gompertz model with high correlation coefficient （R^2〉0.99）. Furthermore, the substrate induction test indicates that DEP-induction can apparently shorten the lag phase and enhance the degradation rate. This work highlights the potential of this isolate for bioremediation of phthalates-contaminated environments.

关 键 词：BIODEGRADATION diethyl phthalate KINETICS Acinetobacter sp. river sludge 

分 类 号：X172[环境科学与工程—环境科学]