人骨髓间充质干细胞在大鼠体内转化为肝样细胞的实验研究  

Differentiation of HMSCs into hepatocyte-like cells in Fischer 344 rats in vivo

作　　者：陈建锋[1] 高毅[2] 宫林[1] 潘明新[2] 蒋泽生[2] 

机构地区：[1]解放军第401医院肝胆外科,山东青岛266071 [2]南方医科大学珠江医院肝胆外科,广州510282

出　　处：《山东大学学报（医学版）》2010年第9期124-126,130,共4页Journal of Shandong University:Health Sciences

摘　　要：目的探讨诱导人骨髓间充质细胞在大鼠肝损伤模型中转分化为肝样细胞的可能性。方法建立肝叶切除和亚致死量照射两种肝脏损伤模型,植入人间充质干细胞,检测大鼠谷丙转氨酶、总胆红素和凝血酶原时间的变化;聚合酶链反应检测大鼠肝脏内人特异性DNA序列Alu-sx。结果造模后行干细胞移植组大鼠肝功能均有明显改善。聚合酶链反应显示各干细胞移植组大鼠肝脏中均能同时检测人特异性DNA序列Alu-sx和大鼠特异性DNA序列Sox11。结论人骨髓间充质干细胞能在肝损伤大鼠肝脏内存活,表达人特异性抗原,并能在肝损伤大鼠肝脏内分化为具有肝功能的肝样细胞,有助于肝损伤大鼠肝功能恢复。Objective To investigate the possibility of inducing human bone marrow mesenchymal stem cells（HMSCs） to differentiate into hepatocyte-like cells in liver injury rat models.Methods HMSCs were induced to differentiate into hep-atocyte-like cells after being transplanted through the portal vein into the injured Fischer 344 rats which previously under-went partial hepatectomy （PH） or whole body irradiation （IR） .The hepatic metabolic functions were measured by deter-mining the alanime transaminase（ALT） ,total bilirubin（TBiL） and prothrombin time（PT） after model-building.Expres-sion of the human specific DNA fragment Alu-sx was detected in the liver of Fischer 344 rats by PCR.Results The he-patic metabolic functions of the rats in cell transplantation groups were apparently better than in the control group.Expres-sions of human specific DNA fragment Alu-sx and rat specific DNA fragment Sox11 were positive in the livers of cell trans-plantation groups.Conclusion HMSCs can survive,differentiate into functional hepatocyte-like cells and express the hu-man specific antigen in injured livers of Fischer 344 rats,contributing to the recovery of liver functions.

关 键 词：骨髓间充质千细胞 细胞分化 肝样细胞 肝部分切除术 大鼠 

分 类 号：R73-35[医药卫生—肿瘤]