HA14-1联合环磷酰胺调控肺癌小鼠Bcl-2、Bax蛋白的表达  

HA14-1 Combined with Cyclophosphamide Modulates the Expression of Bcl-2 and Bax in Lung Cancer in Mice

作　　者：谭明旗[1] 孙惠梅[1] 边明艳[1] 

机构地区：[1]中国医科大学附属盛京医院第二呼吸内科,沈阳市110004

出　　处：《中国肿瘤临床》2010年第19期1086-1088,1093,共4页Chinese Journal of Clinical Oncology

基　　金：辽宁省教育厅2006~2007年度高等学校科研项目基金资助(编号:20060965)

摘　　要：目的:探讨HA14-1联合环磷酰胺对肺癌小鼠Bcl-2、Bax蛋白的表达。方法:建立小鼠肿瘤模型,分为生理盐水组、HA14-1组、环磷酰胺组和HA14-1+环磷酰胺组,应用Western blot和免疫组化方法检测Bcl-2、Bax的蛋白表达。结果:HA14-1组、环磷酰胺组、HA14-1+环磷酰胺组较生理盐水组Bcl-2表达减少、Bax表达增加(P<0.05),HA14-1+环磷酰胺组较单一的HA14-1组或环磷酰胺组Bcl-2表达明显减少、Bax表达明显增加(P<0.05)。结论:HA14-1可以通过抑制Bcl-2的表达,增加Bax的表达,从而促进肿瘤细胞的凋亡,同时增强环磷酰胺的抗肿瘤特性。Objective： To explore the effect of HA14-1 combined with cyclophosphamide （CTX） on the expression of Bcl-2 and Bax proteins in lung cancer in mice. Methods： Mouse models of lung carcinoma were established and divided into 4 groups： the HA14-1 group, the CTX group, the combination HA14-1＋CTX group and the normal saline group used as the control. Western blot and immunohistochemistry were applied to detect the expression of Bcl-2 and Bax. Results： The HA14-1 group, the CTX group and the HA14-1＋CTX group had lower Bcl-2 protein expression and higher Bax expression compared with the control group （P〈0.05）. The HA14-1＋CTX group had the lowest Bcl-2 protein expression and the highest Bax protein expression （P〈0.05）. Conclusion： HA14-1 can promote tumor cell apoptosis and enhance the efficacy of CTX chemotherapy by downregulating Bcl-2 expression and upregulating Bax expression.

关 键 词：HA14-1 Bcl-2 BAX WESTERN BLOT 免疫组化 

分 类 号：R734.2[医药卫生—肿瘤]