E2F3及C-myc在膀胱移行细胞癌组织中的表达及其相关性研究  

Expression of E2F3 and c-Myc in Tissue of Bladder Transitional Cell Carcinoma and Its Significance

作　　者：胡海龙[1] 韩瑞发[1] 孙岩[1] 刘利维[1] 任海林[1] 梁恩利[1] 王春阳[1] 吴长利[1] 

机构地区：[1]天津市泌尿外科研究所,天津市重点实验室,天津市300211

出　　处：《中国肿瘤临床》2010年第19期1094-1096,1101,共4页Chinese Journal of Clinical Oncology

基　　金：天津市科技支撑计划重点项目(编号:07ZCGYSF05200);天津市高等学校科技发展基金计划项目(编号:20060122);国家自然科学基金项目(编号:30700834);天津市卫生局科技基金项目(编号:09KZ90);天津市应用基础及前沿技术研究计划基金资助(编号:09JCYBJC27800)~~

摘　　要：目的:探讨E2F3及C-myc在膀胱癌组织中表达的相互关系及其与膀胱癌恶性生物学行为之间的关系。方法:应用免疫组织化学方法检测不同病理分期及组织分级的BTCC标本和正常膀胱黏膜中E2F3及C-myc蛋白的表达情况,应用免疫组化自动分析系统进行分析,结果以积分光密度表示。结果:膀胱移行细胞癌组织中E2F3及C-myc蛋白的表达阳性率明显高于正常膀胱黏膜,两组间有显著性差异(P<0.05),同时E2F3的表达率与膀胱癌的分级和分期密切相关(P<0.05;P<0.01);C-mvc的表达率与膀胱癌的分级和分期密切相关(P<0.01;P<0.01)。膀胱移行细胞癌中E2F3与C-myc的表达呈正相关。结论:E2F3及C-myc与膀胱癌的恶性程度密切相关,同时二者之间的表达密切相关,二者不仅可以作为膀胱癌的诊断与预后指标,而且为以E2F3为靶点的膀胱癌的基因治疗提供理论依据。Objective： To detect the expression of E2F3 and c-Myc proteins in bladder transitional cell carcinoma （BTCC） tissue and normal bladder epithelial tissue and to analyze the correlation of E2F3 and c-Myc expression with the biological behaviors of BTCC. Methods： Immunohistochemistry was used to detect the expression of E2F3 and c-Myc in BTCC tissue （n=64） and normal bladder mucosa （n=10）. Immunohistochemical results were analyzed with Image-Pro Plus software and the corresponding results were reported as integrated optical densities （IOD）. Results： The expression of E2F3 and c-Myc in BTCC tissue was higher than that in normal bladder mucosa. The expression of E2F3 and c-Myc was correlated with pathological grade and clinical stage （P〈0.05, P〈0.01; P〈0.01, P〈0.01）. Immunohistochemistry indicated that the IOD of E2F3 and c-Myc in BTCC tissue was significantly higher than that in normal bladder mucosa （P〈0.01, P〈 0.01）. E2F3 expression was correlated with c-Myc expression （P〈0.01）. Conclusion： E2F3 and c-Myc protein levels can be used as diagnostic and prognostic indices of BTCC. This study provides a primary theoretical basis for gene target therapy of BTCC.

关 键 词：E2F3 C-MYC 膀胱移行细胞癌 

分 类 号：R737.14[医药卫生—肿瘤]