牛磺酸对内毒素血症幼鼠肠屏障功能障碍的保护作用  

The protective effect of taurine on intestinal barrier function in rats with endotoxemia

作　　者：连淑君[1] 王琳琳[1] 唐清[1] 陈萍[1] 单庆文[1] 王华[1] 云翔[1] 

机构地区：[1]广西医科大学第一临床医学院儿科,广西南宁530021

出　　处：《临床儿科杂志》2010年第10期950-954,共5页Journal of Clinical Pediatrics

基　　金：广西壮族自治区科技厅自然科学基金项目资助(No.桂科自0640086)

摘　　要：目的探讨牛磺酸在内毒素血症所致肠黏膜损伤中的作用。方法腹腔注射大肠杆菌脂多糖(5mg/kg)以建立幼鼠内毒素血症动物模型。健康18日龄Wistar大鼠72只,随机分为内毒素血症组(LPS组)、牛磺酸干预组(TAU组)、正常对照组(NS组)。LPS组和TAU组制模后分为2﹑6﹑12﹑24h4个亚组,每亚组8只,在4个时间点分别取肠黏膜标本制备石蜡切片和组织匀浆,光学显微镜及电子显微镜下观察小肠组织病理改变,免疫组织化学染色技术检测NF-κB活化水平,ELISA法检测肠组织匀浆TNF-α和IL-6。结果 LPS组病理结果示微绒毛萎缩、脱落,线粒体严重肿胀,呈空泡变性,染色质浓缩分布于核周边,核仁碎裂消失,凋亡细胞多见,细胞结构紊乱、异常;小肠组织NF-кB活化水平检测结果示各时间点的核阳性细胞比率均较NS组高,以2h最明显;2h时小肠匀浆TNF-α﹑IL-6含量明显高于NS组。TAU可以明显降低NF-кB活化和TNF-α、IL-6组的水平,肠道黏膜损伤较LPS组减轻。结论 TAU可通过减少肠道NF-κB活化,降低炎性因子TNF-α、IL-6水平,保护肠黏膜屏障功能。Objective To investigate the role of taurine in protecting intestinal barrier function in endotoxemia. Methods The rats endotoxemia model was made by injection of lipopolysaccharide 5 mg/kg into abdominal cavity. The healthy 18-day Wistar rats were selected and divided into three groups, lipopolysaccharide group （LPS group）, taurine group （TAU group） and control group （NS group）. The rats in LPS group and TAU group were further divided into 2 h, 6 h, 12 h and 24 h sub-groups and each sub-group had eight rats. Intestinal mucosa membranes were collected and made paraffin section or homogenate. The pathological characteristics of the small intestine were observed under light and electronic microscope. The level of TNF-α and IL-6 were detected by ELISA. The activation of NF-κB was evaluated by immunohistochemistry. Results Pathological examination showed that, in LPS group, the intercellular space became broaden, microvilli villous were disarranged and atrophied, mitochondria swelled seriously with vacuolar degeneration, chromatin condensed around the nuclear, nucleoli fragmented and disappeared, a large number of apoptosis cells were observed, the structure of cells became disorder and abnormal. The activations of NF-κB at each point of time were higher in LPS group than in NS group, especially in 2 h sub-group. The level of TNF-α and IL-6 were higher in LPS group than that in NS group, especially in 2 h sub-group. The activations of NF-κB and the level of TNF-α and IL-6 in TAU group were significantly lower in TAU group than that in LPS group at the same time. The damages of intestinal mucosa membranes were slighter in TAU group than that in LPS group. Conclusions Taurine can reduce the activation of NF-κB, and the levels of TNF-α and IL-6 in intestinal mucosa membranes, so that it has the protective effect on intestinal barrier function.

关 键 词：牛磺酸 内毒素血症 肠屏障功能障碍 核因子-κB 肿瘤坏死因子α 白介素6 大鼠 

分 类 号：R725.7[医药卫生—儿科]