HepaCAM对肾癌786-0细胞Caspase-3活性和表达的影响  

Effect of hepaCAM on the Activity and Expression of Caspase-3 in Renal Cell Carcinoma 786-0 Cells

作　　者：张巧琳[1] 罗春丽[1] 颜令[1] 

机构地区：[1]重庆医科大学医学检验系临床检验诊断学教育部重点实验室重庆市重点实验室,重庆400016

出　　处：《生物技术通报》2010年第11期142-145,共4页Biotechnology Bulletin

摘　　要：探讨肝细胞黏附分子(hepatocyte cell adhesion molecule,hepaCAM)在肾癌786-0细胞中不同表达水平对其凋亡及Caspase-3活性和表达的影响。将携带hepaCAM基因的重组质粒瞬时转染786-0细胞,RT-PCR鉴定hepaCAM基因在786-0中的表达;FCM检测细胞凋亡情况;分光光度计结合Western印迹法检测hepaCAM表达与Caspase-3活性及蛋白水平表达的关系。结果显示,上调hepaCAM基因表达能诱导肾癌786-0细胞凋亡,能上调Caspase-3活性及其蛋白水平且与hepaCAM表达水平呈正相关。上述结果表达,hepaCAM可能通过调节Caspase-3凋亡通路诱导肾癌786-0细胞凋亡。The study was to investigate the effect of different expression of hepatocyte cell adhesion molecule on apoptosis and the activity and expression of Caspase-3 in renal cell carcinoma 786-0 cells.The vector containing hepaCAM gene was transiently transfeceted into 786-0 cells,and the expression of hepaCAM gene in 786-0 cells was confirmed by RT-PCR.The cell apoptosis of 786-0 cells was analyzed by FCM.The relationship between the expression of hepaCAM and the activity and expression of Caspase-3 was tested by western bolt conbined with spectrophotometer.Results indicated that unregulating the expression of hepaCAM gene can induce apoptosis in renal cell carcinoma 786-0 cells,and the activity and expression of Caspase-3 can be upregulated markedly which positive correlated with the expression of hepaCAM.Therefore,hepaCAM can induce apoptosis possibly through Caspase-3 dependent pathway in renal cell carcinoma 786-0 cells.

关 键 词：hepaCAM 肾癌 凋亡 CASPASE-3 

分 类 号：R737.11[医药卫生—肿瘤]