多疣壁虎DNA含量的测定(英文)  

Determination of DNA content of Gekko japonicus by flow cytometry

作　　者：刘炎[1] 李静[1] 顾云[1] 沈宓[1] 王勇军[1] 刘梅[1] 

机构地区：[1]江苏省神经再生重点实验室南通大学,南通226001

出　　处：《安徽农业大学学报》2010年第4期609-613,共5页Journal of Anhui Agricultural University

基　　金：国家自然科学基金项目(30600172);江苏省教育厅自然科学基金(07KJB180097和09KJA180005)共同资助~~

摘　　要：采用流式细胞术方法测定多疣壁虎(Gekko japonicus)DNA含量(C值)。结果表明,多疣壁虎的C值为2.928 pg.C-1,计算其基因组大小约为2.86×109bp,雌性和雄性壁虎的C值分别为2.934 pg和2.923 pg。数据统计经独立样本t检验性别间无显著差异。相同方法测定了另外两种爬行动物北草晰(Takydromusseptentrionalis)和原尾壁虎(Hemidactylus bowringii)的C值,分别是(2.10±0.03)pg.C-1和(2.04±0.03)pg.C-1,并计算其基因组大小,北草晰为2.054×109bp,原尾壁虎为1.995×109bp。The present study aims to investigate the DNA content（C-value） of Gekko japonicus by flow cytometry.The result showed that the C-value of Gekko japonicus is 2.928 pg·C-1 with the calculated genome size approximately of 2.86×109 bp.The C-values for female and male are 2.934 pg and 2.923 pg,respectively.Independent-samples T test indicated that the C-values of different genders have no significant difference.Meantime,we observed the C-values of two other reptile species,and found that C-values of Takydromus septentrionalis and Hemidactylus bowringii are（2.10±0.03） pg·C-1 and（2.04±0.03） pg·C-1.By this means the calculated approximate genome size of Takvdromus septentrionalis is 2.054×109 bp and that of Hemidactylus bowringii is 1.995×109 bp.

关 键 词：流式细胞术 DNA含量(C值) 多疣壁虎 爬行动物 

分 类 号：Q959.62[生物学—动物学]