^(60)Coγ射线辐照马铃薯适宜剂量与效应研究  

OPTIMUM DOSE AND EFFECTS OF ^(60)Co-γ IRRADIATION ON POTATO

作　　者：杨先泉[1,2] 王翠[1,2] 赵勤[1] 刘勇强[3] 

机构地区：[1]四川农业大学农学院,四川雅安625014 [2]四川农业大学马铃薯研究中心 [3]四川省原子能研究院,四川成都610066

出　　处：《核农学报》2010年第5期917-921,共5页Journal of Nuclear Agricultural Sciences

基　　金：四川省科技厅攻关项目(05-NG001-021-2);四川省教育厅重点项目(2005A025);四川省委组织部"天府英才"和四川省农业厅"马铃薯提升计划"

摘　　要：采用不同剂量(27.0、31.5、36.0和40.5Gy)60Coγ射线照射3个马铃薯品种(系)S6-3、S6-4和川芋5号块茎,分析辐照剂量对田间成苗率、出苗动态及几个叶片生理指标的影响。结果表明:辐照对供试品种(系)致死作用剂量效应明显,适宜剂量为31.7～39.9Gy。辐照降低出苗率、推迟出苗期、延长出苗持续期,品种(系)间存在敏感性差异;辐照当代(M1)田间未筛选到具有显著形态性状变异的个体。S6-3叶片生理特征分析表明:本试验剂量范围内,辐照对马铃薯当代植株表现的影响主要是损伤和抑制效应。分析认为:在马铃薯块茎辐射诱变时应采用较低的剂量率,并应对后代(VM2)株系进行更细致的形态性状、生理生化特性与分子生物学鉴定筛选。Tubers of 3 potato varieties were irradiated by of ^60Co γ-irradiation.Effects of irradiation on survival rate,physiological characteristics of seedling stage were analyzed.The results showed that irradiation had remarkable lethal effect on 3 tested varieties,and optimum doses of irradiation were 31.7 ～ 39.9Gy.Irradiation affected seedling development by decreasing seedling rate,delaying seedling stage and extending seedling period.There were different sensitivities among varieties.No distinct morphologic variant was observed in M1.Analyses of physiological characteristics of S6-3 leaves indicated that effect of irradiation on M1 plant was damage and/or suppression.It is suggested that lower dose rate might be applied for mutation inducing;and precisely morphological,physiological,biochemical and molecular biological investigation should be carried out to screen mutant within offspring（VM2） lines.

关 键 词：马铃薯 ^60Coγ 辐照 半致死剂量 生理效应 

分 类 号：S532[农业科学—作物学] S124