草原红牛IGFBP-5基因外显子多态性及对胴体性状的影响  

SNP analysis of IGFBP-5 gene exons and relationship with carcass weight in red steppes

作　　者：芦春艳[1] 杨润军[1] 姜昊[2] 张金玉[1] 张国梁[3] 赵玉民[3] 赵志辉[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]吉林大学实验动物中心,吉林长春130062 [3]吉林省农业科学院畜牧分院,吉林公主岭136100

出　　处：《中国兽医学报》2010年第7期1005-1008,共4页Chinese Journal of Veterinary Science

基　　金：国家科技支撑计划资助项目(2007BAD55B01);国家"863"计划资助项目(2008AA101010)

摘　　要：为揭示草原红牛IGFBP-5基因外显子多态性与胴体性状的相关性,采用PCR-SSCP及PCR-RFLP两种方法分别对58头草原红牛群体该基因外显子多态性进行了检测,并对检测到的突变位点用SPSS软件分析其与胴体性状的相关性。结果表明:IGFBP-5基因第3外显子在191bp处存在G(A)突变,该基因多态性与宰前活重、胴体重呈显著相关,BB型显著高于AA型(P>0.05);对其他性状的影响差异不显著。通过研究IGFBP-5基因外显子的多态性,为草原红牛的选种选育提供实验依据。This experiment was conducted to detecte SNP of IGFBP-5 gene exons and the relationship with carcass weight in red steppes.The polymorphism of IGFBP-5 exons in red steppes was detected by PCR-SSCP and PCR-RFLP.And then the genetic correlations between genotypes and performances was obtained by SPSS.The results indicated that there was one single nucleotide mutation G（A） in the 191 bp of exon 3 of IGFBP-5 gene;And the genetic polymorphisms significantly correlated with living weight.And living weight of BB genotype was higher than AA genotype（P0.05）.Other traits were not significantly affected.The research laid a foundation for the future studies of the biological function of IGFBP-5 gene genetic in red steppes and a theoretical basis for breeding of red steppes.

关 键 词：中国草原红牛 IGFBP-5 PCR-SSCP PCR-RFLP 胴体性状 

分 类 号：S813.3[农业科学—畜牧学]