鸡传染性喉气管炎病毒gB基因重组DNA疫苗的构建与鸡IL-18联合免疫试验  

Construction and combined immunization effect of the recombinant DNA vaccine expressing ILTV major antigen epitope gene and chicken IL-18

作　　者：邵攀峰[1,2,3] 陈红英[1,2] 崔保安[1,2] 王振亚[1,2] 宋亚鹏[1,2] 刘金朋[1,2] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]河南省动物性食品安全重点实验室,河南郑州450002 [3]杭州荐量兽用生物制品有限公司,浙江杭州310018

出　　处：《中国兽医学报》2010年第10期1296-1300,共5页Chinese Journal of Veterinary Science

摘　　要：本研究构建了编码ILTV糖蛋白gB基因的重组DNA疫苗pgB,为测定该DNA疫苗与鸡IL-18联合免疫的试验效果,将pgB、pIL-18分别以单独和联合的方式免疫21日龄SPF雏鸡,免疫后每周抽取外周血及外周抗凝血,应用ELISA和流式细胞仪分别检测免疫鸡的体液免疫及细胞免疫水平。二免疫后第3周用100 ELD50ILTV强毒进行攻击。结果显示,pgB和pgB与pIL-18联合免疫组均能诱导鸡产生特异性的体液免疫及细胞免疫应答,其中以pgB与pIL-18联合免疫组的T淋巴细胞亚群CD3+、CD3+CD4+、CD3+CD8+明显高于单独pgB免疫组(P<0.01),87%抵抗ILTV强毒的致死性攻击。A plasmid DNA vaccine pgB expressing gB protein of infectious laryngotracheitis virus（ILTV） was constructed,to evaluate the prophylactic efficacy of the DNA vaccine.3-week-old chickens were immunized with pgB,pIL-18 and pgB.After immunization,peripheral blood lymphocytes were detected by flow cytometry and the sera were collected to examine antibodies by ELISA each week.The chickens were challenged with 100 ELD50ILTV on week 3 after immunization.The results indicated that chickens vaccinated with pIL-18 and pgB had an insignificant increase in the percentage of CD3＋、CD3＋CD4＋、CD3＋CD8＋ subgroups of peripheral blood.Chickens vaccinated with pIL-18 and pgB were 87% protected against challenge with ILTV.The results demonstrate that the combined immunization can elicit cell immune response remarkably and improves the protective effect against ILTV infection.

关 键 词：传染性喉气管炎 DNA疫苗 糖蛋白B 鸡IL-18 

分 类 号：S852.65[农业科学—基础兽医学]