“小粒黄”大豆子叶节丛生芽的诱导  

Induction of Multiple Shoots from Cotyledonary Nodes of "Xiaolihuang" Soybean

作　　者：曹荣[1] 芦琳琳[1] 朱保葛[2] 朱木兰[3] 薛仁镐[1] 

机构地区：[1]青岛农业大学生命科学学院,山东青岛266109 [2]中国科学院遗传与发育生物学研究所,北京100101 [3]中国科学院上海生命科学研究院植物生理生态研究所,上海200032

出　　处：《大豆科学》2010年第5期747-750,共4页Soybean Science

基　　金：转基因生物新品种培育重大专项资助项目(2009ZX08010-009B)

摘　　要：为建立大豆子叶节高频再生体系,以"小粒黄"大豆子叶节为外植体,研究了萌发天数、6-BA及TDZ浓度对大豆子叶节丛生芽诱导的影响。结果表明:随着萌发天数的增加丛生芽诱导率下降,萌发7 d的子叶节外植体对丛生芽诱导率最高,达到82.6%。附加不同浓度6-BA和TDZ的1/2MSB5的基本培养基上均能诱导出丛生芽,但丛生芽诱导率也不同,以1.5 mg.L-16-BA和0.2 mg.L-1TDZ配比时,丛生芽诱导率最高,达到85.7%。To establish a high frequency regeneration of soybean, the effects of germination day, 6-BA and TDZ concentrations on the induction of multi-shoot were studied using cotyledonary nodes of＂Xiaolihuang＂ as explants in this study. The results showed that the days of germination affected the induction rate of multi-shoot. The highest induction rate of multi-shoot was obtained from the explants of 7-day germinated cotyledonary nodes, which reached 82.6%. With the increasing of the germi- nation day, the induction rate of multi-shoot decreased. Although multi-shoot can be induced from the 1/2MSB5 mediums supplemented with different concentrations of the 6-BA and TDZ, the best induction rate of muhi-shoots was occurred when combining of 1.5 mg · L-I 6-BA with O. 2 mg· L- 1 TDZ, which reached 85.7%.

关 键 词：大豆 子叶节 丛生芽 6-BA TDZ 

分 类 号：S565.1[农业科学—作物学]