刺梨花粉萌发的培养基筛选  

Screening for Culture Medium for Rose roxburghii Pollen Germination

作　　者：程秀枝[1] 陈红[1,2] 

机构地区：[1]贵州大学喀斯特山地果树资源研究所,贵州贵阳550025 [2]贵州省果树工程技术研究中心,贵州贵阳550025

出　　处：《贵州农业科学》2010年第10期28-30,共3页Guizhou Agricultural Sciences

基　　金：贵州省省长基金资助项目"贵州特色果树育种新材料的创制"(黔省专合字2007-18);贵州省果树学科科技创新人才团队建设项目[黔科合人才团队(2008)88007];贵州省特色农业产业人才培养基地建设项目;贵州省果树工程技术研究中心建设项目[黔科合农G字(2007)4001]

摘　　要：为筛选刺梨花粉萌发的最佳培养基,研究了不同蔗糖浓度、硼酸浓度和Ca2+浓度对刺梨花粉萌发率的影响,进一步探讨了不同品种在最佳培养基上花粉萌发率的差异。结果表明,最佳培养基组合为200g/L蔗糖+20mg/LH3BO3+20mg/L CaCl2+10g/L琼脂;不同刺梨品种的萌发率差异较大,贵农1号、2号、5号、6号、7号花粉的萌发率分别为74.8%、54.51%、83.29%、70.92%和80.48%。Effects of different concentration of sucrose,boric acid and Ca^2＋ on germination rate were studied by in vitro culture to screen out the optimum culture medium for Rose roxburghii pollen germination and further explored germination rate difference of different cultivars on the optimum culture medium.The results showed that the optimal culture medium was 200 g/L sucrose ＋ 20 mg/L boric acid ＋ 20 mg/L CaCl2 ＋10 g/L agar.The Pollen germination rates of Guinong 1,Guinong 2,Guinong 5,Guinong 6 and Guinong 7 was 74.8%,54.51%,83.29%,70.92% and 80.48%respectively,which indicated that there is great difference of germination rate among series cultivars.

关 键 词：刺梨 花粉 培养基 品种 萌发率 

分 类 号：S668.9[农业科学—果树学]