一株发酵乳杆菌培养条件的优化  

Optimization of growth conditions for lactobacillus fermentum

作　　者：洪梅[1] 刁其玉[1] 闫贵龙[2] 屠焰[1] 张乃锋[1] 姜成钢[1] 

机构地区：[1]中国农业科学院饲料研究所 [2]河北北方学院

出　　处：《饲料工业》2010年第19期26-29,共4页Feed Industry

基　　金：国家"十一五"科技支撑项目"优质青贮和半干青贮技术研究及微生物添加剂开发"(2006BAD04A04-05)

摘　　要：为更好地提高青贮饲料的质量,研究对分离自中国青贮窖中的一株乳酸菌BLF01进行了分子学鉴定,结果显示为发酵乳杆菌。然后通过单因素试验设计研究了培养基组成(碳源、氮源)和培养条件(温度、接种量、起始pH值)对BLF01乳酸菌生长繁殖的影响。结果表明,其最适碳源是乳糖,最佳氮源是酵母粉;最佳培养条件是起始pH值6.5、培养温度35℃、接种量1%,可以考虑将BLF01作为青贮接种剂使用。To improve the quality of silage and to decrease costs of microbial inoculants,culture conditions were optimized for the growth of a strain BLF01 of lactobacillus fermentum. The critical medium conditions including carbon source,nitrogen source,initial pH,temperature and inoculum size were identified by the one-factor-at-a-time approach. The optimal carbon source and nitrogen source were found to be lactose and yeast powder,respectively. The optimal pH,temperature and inoculum size for culture of BLF01 were 8.0,35 ℃ and 1%,respectively. Lactobacillus fermentum BLF01 can be used as a microbial inoculant in silage,but the effect remains to be clarified.

关 键 词：青贮饲料 发酵乳杆菌 培养基优化 

分 类 号：S816.3[农业科学—饲料科学]