AFLP技术的优化及在三倍体银鲫DNA指纹图谱中的应用  

Optimized AFLP Technology and Its Application in DNA Fingerprinting of Triploid Silver Crucian Carp(Carassius auratus gibelio Bloch)

作　　者：韩启霞[1,2] 高国强[1,2] 刘春雷[1,2] 徐丽华[1,2] 池炳杰[1,2] 梁利群[1] 

机构地区：[1]中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《水产学杂志》2010年第3期16-20,共5页Chinese Journal of Fisheries

基　　金：国家高技术研究发展计划(863计划)(2007AA10Z186)

摘　　要：扩增片段长度多态性(Amplified Fragment Length Polymorphism,AFLP)技术具有多态性丰富,所需DNA量少,灵敏度高等优点,但流程繁琐,花费时间长。本文根据AFLP技术的特点,在酶切-连接和聚丙烯酰胺凝胶的制备,这两方面做了很大改进。采用优化的AFLP技术分析三倍体银鲫的DNA指纹图谱,不仅缩短了实验所需时间,而且获得了稳定清晰的扩增带型。AFLP（Amplified Fragment Length Polymorphism） is characterized not only by the merits of highly polymorphoism,less DNA usage and higher sensitivity,but by the drawbacks of tedious procedure and time-consuming.In this paper,nearly each step of AFLP was optimized,especially in gel-making.DNA fingerprinting of triploid silver crucian carp（Carassius auratus gibelio Bloch） showed that using optimized AFLP can not only save much time but obtain reproductive results.

关 键 词：AFLP标记 聚丙烯酰胺凝胶电泳 银鲫 

分 类 号：Q953[生物学—动物学]