SOE-PCR技术在洛伐他汀九酮合成酶基因克隆中的应用  

Application of SOE-PCR in the Lovastatin Nonaketide Synthase Gene Cloning

作　　者：黄卓[1] 李东栋[1] 张琳[1] 严珊[1] 

机构地区：[1]海南大学材料与化工学院,海南海口570228

出　　处：《海南师范大学学报（自然科学版）》2010年第3期300-305,共6页Journal of Hainan Normal University（Natural Science）

基　　金：国家自然科学基金(30560092)

摘　　要：为了验证SOE-PCR技术在洛伐他汀九酮合成酶基因(LOVB)克隆中的应用,以土曲霉基因组DNA为模板,设计4对引物,分别扩增LOVB的4个外显子,并按一定的顺序,利用SOE-PCR技术将其一一串联起来,形成LOVB①-④的cDNA序列.结果表明:重叠延伸PCR成功扩增出了LOVB①-④的cDNA序列,大小1.3kb左右,测序结果和NCBI中登录号为AF151722.1的洛伐他汀九酮合成酶基因LOVB①-④的cDNA序列比对,同源性为98.5%.In order to verify the application of SOE PCR in the lovastatin nonaketide synthase gene （LovB） cloning,4 couples of primers were designed to amplify 4 exons of LovB, after which the exons can be connected by overlapping PCR one by one to form the cDNA sequence of LovB ①-④.The results indicated that the exons of LovB①-④ with the size of 1300bp was amplified successfully by overlapping PCR,the sequencing result was blasted with the LovB cDNA sequence titled AF151722.1 in the NCBI, which showed the 98.5% homoeology.

关 键 词：重叠延伸PCR 洛伐他汀 聚酮合成酶 他汀类药物 

分 类 号：R392.11[医药卫生—免疫学]