维A酸结合蛋白Ⅰ折叠/伸展过程的氢/氘交换质谱研究  

Hydrogen/Deuterium Exchange Mass Spectrometry Study on the Folding/Unfolding Process of Cellular Retinoic Acid-Binding Protein Ⅰ

作　　者：张琳[1] 谢建英[1] 

机构地区：[1]广东海洋大学理学院,广东湛江524088

出　　处：《质谱学报》2010年第5期302-305,共4页Journal of Chinese Mass Spectrometry Society

摘　　要：氢/氘交换质谱通过测定蛋白骨架中的氢(氘)与溶剂中氘(氢)的相互交换作用来研究蛋白的结构信息。采用氢/氘交换技术结合傅里叶变换离子回旋共振质谱研究氘代维A酸结合蛋白Ⅰ与溶剂之间的氢/氘交换,发现在不同的pH值下,该蛋白的氢/氘交换具有不同的交换机理和交换速率,由其交换速率可估计不同交换时间蛋白骨架中残留的未交换的氢或氘的数目,确定蛋白骨架在溶剂中的暴露程度,得到蛋白的结构信息。The structural information of proteins was studied with hydrogen/deuterium exchange mass spectrometry by testing the hydrogen（deuterium） on protein bond exchange with the deuterium（hydrogen） in solvents.Utilizing hydrogen/deuterium exchange technique combined with Fourier transform ion cyclotron resonance mass spectrometry,the hydrogen/deuterium exchange in deuterium-cellular retinoic acid-binding protein Ⅰ and its solvents was investigated.At different pH,the different exchange mechanism and different exchange speed are found for this protein.From the speed of the hydrogen/deuterium exchange,the number of unexchangeing hydrogen or deuterium at different exchange time remained in protein can be estimated,confirming the extent of the protein bond exposure to the solvents to obtain the structural information of protein.

关 键 词：氢/氘交换 质谱 维A酸结合蛋白 

分 类 号：O657.63[理学—分析化学]