微米红参的制备及含量测定  

Preparation and Content Determination of Micron Red Ginseng

作　　者：杨凯[1] 石莹[1] 李镐[1,2] 郭建鹏[1,2] 

机构地区：[1]延边大学药学院,吉林延吉133000 [2]长白山生物资源与功能分子教育部重点实验室,吉林延吉133002

出　　处：《中国实验方剂学杂志》2010年第14期17-19,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：吉林省科技发展计划项目(200705422);吉林省教育厅"十一五"科学技术研究项目(2009-18)

摘　　要：目的:优化微米红参的制备工艺,测定其主要人参皂苷含量。方法:采用单因素法对影响红参微米化因素进行筛选,以平均粒径和粒度分布为考察指标,优化微米红参化生产的最佳工艺;采用高效液相色谱法测定人参皂苷Rg1,Re,Rb1的含量。结果:最佳工艺为风袋长度20 cm,粉碎时间为40 min,单次进料量为500 g;测得样品中人参皂苷Rg1,Re,Rb1的含量分别2.06,1.77,2.41 mg.g-1,微米红参人参皂苷含量测定显著高于红参普通粉与切片。结论:微米红参制备工艺合理、稳定;建立的含量测定方法适用于微米红参;微米红参利于人参皂苷成分溶出。Objective：The preparation process optimization of microns red Ginseng was performed with de-tection of ginsenoside.Method：The single factor law on the factors affected red Ginseng microns in diameter screening,particle size distribution to optimize the production of red Ginseng microns optimum technology.The gin-senoside Rg1,Re and Rb1 were determined by HPLC.Result：The best process was as follows：the wind bag length was 20 cm,crushing time 40 min into the charge for a single 500 g;the concent of ginsenoside Rg1,Re,Rb1 were 20.6,17.7,24.1 mg.g-1 respectively.Ginsenosides from micron Red Ginseng was significantly higher than normal powder and sliced Red Ginseng.Conclusion：Micron Red Ginseng preparation technology was stable.The content determination method established can be used for determination of ginsenosides composition in micron Red Ginseng.

关 键 词：超微粉碎 人参皂苷 高效液相 

分 类 号：R283.6[医药卫生—中药学]