大黄鱼肌肉生长抑制素基因外显子Ⅱ遗传多态性分析  

Gentic polymorphism analysis of exon Ⅱ in MSTN gene from large yellow croaker,Pseudosciaena crocea

作　　者：杨斌[1] 薛良义[1] 叶秀丽[1] 董小敬[1] 

机构地区：[1]宁波大学生命科学与生物工程学院,浙江宁波315211

出　　处：《海洋通报》2010年第5期554-559,共6页Marine Science Bulletin

基　　金：国家863项目(2006AA10A405);国家自然科学基金(30871916)

摘　　要：肌肉生长抑制素是控制动物骨骼肌生长发育的重要细胞因子,采用PCR-SSCP和测序方法分析了150尾大黄鱼样本肌肉生长抑制素基因(myostatin)外显子Ⅱ的单核苷酸多态性,结果发现,外显子Ⅱ中有10个多态位点,分别是29、131、206、261、231、243、265、273、297和300位。其中131位点T→A突变引起相应的氨基酸由L→Q,265位点T→C突变引起相应氨基酸由C→R,261位点A→G突变引起相应氨基酸由S→G,231位点的T→C突变引起相应氨基酸由W→S,273位点的C→T突变引起相应的氨基酸由R→W,297位点的A→G突变引起相应氨基酸由I→V,243位点的G→A突变引起相应氨基酸由D→N,300位点的G→A突变引起相应氨基酸由E→K。29位点A→G和206位点C→A均属于同义突变。存在6种基因型,他们的基因频率分别是0.57、0.23、0.10、0.04、0.02、0.04,该基因位点的杂合度为0.33。Myostatin（MSTN） as an important cytokine controls the growth and development of skeletal muscle in animals.Ten single nucleotide polymorphisms（SNPs） were totally identified in the exon Ⅱof MSTN gene from 150（70 of one-year old and 80 of two-year old） large yellow croakers,Pseudosciaena crocea,individuals by PCR-SSCP and sequencing.The ten polymorphic sites were located at 29（A→G）,131（T→A）,206（C→A）,231（T→C）,243（G→A）,261（A→G）,265（T→C）,273（C→T）,297（A→G） and 300（G→A）.Base mutations at sites 131,265,261,231,273,297,243 and 300 resulted in amino acid substitutions of L→Q,C→R,S→G,W→S,R→W,T→V,D→N and E→K,respectively.However,base mutations at sites 29 and 206 did not cause amino acid substitution.There were six genotypes in the examined population,whose frequency was 0.57、0.23、0.10、0.04、0.02and 0.04,respectively.The heterozygosity of MSTN locus was 0.33.

关 键 词：大黄鱼 肌肉生长抑制素基因 单链构象多态性 生长性状 

分 类 号：Q959.483[生物学—动物学] Q31