莫诺苷对局灶性脑缺血再灌注大鼠皮层IL-1β的影响  

Effects of Morroniside on IL-1β in Rat Cortex with Focal Cerebral Ischemia/Reperfusion

作　　者：艾厚喜[1] 汪莹[1,2] 许栋明[3] 王文[1] 高东明[2] 张丽[1] 李林[1] 

机构地区：[1]首都医科大学宣武医院,北京市100053 [2]辽宁医学院,辽宁锦州市121000 [3]科技部火炬高技术产业开发中心,北京市100038

出　　处：《中国康复理论与实践》2010年第10期928-930,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金："重大新药创制"科技重大专项(2009ZX09103-366);国家自然科学基金项目(30973893);北京市自然科学基金项目(7102077)

摘　　要：目的研究山茱萸有效成分莫诺苷对局灶性脑缺血再灌注大鼠皮层IL-1β的影响。方法用线栓法制备大鼠局灶性脑缺血再灌注模型,缺血30min,再灌注72h。以秋水仙碱为阳性对照药,采用酶联免疫吸附法(ELISA)检测大鼠脑皮层炎症因子IL-1β的变化。结果莫诺苷可剂量依赖性地明显降低由脑缺血再灌引起的IL-1β含量增加(P<0.001)。结论莫诺苷能通过增强大鼠皮层抗炎能力发挥神经保护作用。Objective To investigate the effects of morroniside on IL-1β in rat cortex with focal cerebral ischemia/reperfusion.Methods The animal model was induced by occlusion of middle cerebral artery with suture embolus,ischemia for 30 min,and reperfusion for 72 h in rats. The content of IL-1β was detected with enzyme linked immunosorbent assay（ELISA）.Results Compared with sham group,the content of IL-1β increased obviously in model rat. Compared with model group,morroniside（30 mg/kg,90 mg/kg,270 mg/kg） and colchicine （0.06 mg/kg） decreased the content of IL-1β significantly （P〈0.001）.Conclusion Morroniside may protect the cortex from inflammatory factor IL-1β.

关 键 词：莫诺苷 缺血再灌注 IL-Β 抗炎 神经保护 

分 类 号：R743[医药卫生—神经病学与精神病学]