DC-CIK细胞体外抗淋巴瘤细胞的免疫效应研究  

Investigation for DC-CIK cells' immunoeffects on killing lymphoma cells in vitro

作　　者：艾丽梅[1] 毛淑丹[1] 宋盈盈[1] 

机构地区：[1]辽宁医学院附属第一医院血液科,锦州121001

出　　处：《中国免疫学杂志》2010年第10期898-900,共3页Chinese Journal of Immunology

基　　金：辽宁省教育厅科技研究项目(2008390);辽宁医学院博士启动基金(2009)资助

摘　　要：目的:探讨共培养树突状细胞(Dendritic cell,DC)和细胞因子诱导的杀伤细胞(Cytokine-induced killer cell,CIK),即产生的DC-CIK细胞抗淋巴瘤免疫效应的机制。方法:分离健康人外周血单个核细胞,分别于体外诱导DC和CIK,然后共培养成DC-CIK细胞。分四组:DC组、DC-T组、CIK组和DC-CIK组。对各组分别进行免疫机制研究:测定细胞因子IL-12、IL-17和IFN-γ的分泌量、检测细胞免疫表型和分析对人淋巴瘤细胞株杀伤活性。结果:在DC-CIK组,细胞因子IL-12、IL-17和IFN-γ的分泌量均明显高于其它三组(P<0.05);杀伤活性亦较其它组增强(P<0.05)。CD8+、CD3+/CD56+细胞在DC-CIK组亦明显增加(P<0.05)。结论:DC-CIK细胞抗淋巴瘤免疫效应与分泌大量的细胞因子和产生细胞毒性细胞有关。Objective：To investigate the anti-lymphoma immunoeffects of DC-CIK triggered by cocuhuring dendritic cells and cytokine- induced killer cells. Methods： Mononuclear cells were isolated from. healthy human peripheral blood. DC and CIK were generated in vitro with normal methods, and then cocuhuring the cells at proper ratio to produce DC-CIK cells. Four groups were divided as follows： DC group, DC-T group, CIK group and DC-CIK group. Then the main investigation on immunoeffects included： cytokine and immunophenotyping were detected respectively ,and cytotoxicity of the effectors was analyzed. Results： In DC-CIK group, secretion of cytokine IL-12, IL-17 and IFN-γ was enhanceal. The amount of CD8^＋ and CD3^＋ /CD56^ ＋ cells was all more than those of any other group （all P 〈 0.05）, and the cytotoxicity was also stronger than in any other groups. Conclusion： Immunoefficacy of DC-CIK against lymphoma is relevant to cytokine and cytotoxicity.

关 键 词：DC-CIK细胞 抗淋巴瘤细胞 免疫效应 

分 类 号：R733.4[医药卫生—肿瘤]