乳腺癌患者血清中核因子-κB和CA15-3的检测及临床价值  

Clinical Value of Detection of Serum NF-κB and CA15-3 Expressions in Patients with Breast Cancer

作　　者：李志国[1] 董学君[2] 

机构地区：[1]温州医学院,325035 [2]浙江省绍兴市人民医院分子医学中心,312000

出　　处：《放射免疫学杂志》2010年第5期535-538,共4页Journal of Radioimmanology

基　　金：绍兴市科技计划项目(2009A23005);浙江省医药卫生科学研究基金(2009A208)

摘　　要：目的:探讨乳腺癌患者血清中核因子-κB(NF-κB)和CA15-3的表达水平及其在乳腺癌的诊断、转移和复发诊断中的应用价值。方法:NF-κBELISAkit和i2000免疫化学分析仪检测乳腺癌、乳腺良性肿瘤患者以及正常女性血清中NF-κB、CA15-3的浓度。结果:与正常对照组比较,乳腺癌患者血清中NF-κB、CA15-3的浓度升高,具有统计学差异(P<0.05),乳腺良性肿瘤组与正常对照组比较无明显差异(P>0.05)。乳腺癌临床分期的组间比较,Ⅲ期和Ⅳ期乳腺癌患者比Ⅰ期乳腺癌患者血清中NF-κB、CA15-3的浓度表达高,具有统计学差异(P<0.05),而Ⅱ期与Ⅰ期比较无明显差异(P>0.05);有腋窝淋巴结转移的Ⅱ期、Ⅲ期和Ⅳ期乳腺癌患者血清中NF-κB、CA15-3的浓度比没有腋窝淋巴结转移的同期患者血清表达的浓度高,具有统计学差异(P<0.05);术后1年乳腺癌复发的患者比术后未复发的患者血清中NF-κB、CA15-3的浓度高,存在明显差异(P<0.05),未复发的乳腺癌患者血清NF-κB、CA15-3的浓度同术前血清中的表达有明显的下降,存在统计学差异(P<0.05)。血清NF-κB与CA15-3联检乳腺癌时敏感性可提高到90.6%。结论:NF-κB和CA15-3可以作为乳腺癌的诊断、转移和术后复发监测的指标。Objective To assess the clinical value of detection of serum neuclear factor-κB （NF-κB） and CA15-3 expressions in patients with breast cancer.Methods Serum NF-κB （with ELISA） and CA15-3 （with immunochemistry） expressions were measured in 138 patients with breast cancer （stageⅠ,n =30,stageⅡ,n =51,stage Ⅲ and Ⅳ,n =57） ,40 patients with benign breast disorders,and 35 controls.Results The serum expressions of NF-κB and CA15-3 in patients with breast cancer were significantly higher than those in patients with benign breast disorder and controls （P〈 0.05） ,but there were no significant differences between the values in patients with benign breast disorder and controls （P〈 0.05） .The serum NF-κB and CA15-3 expressions in patients with breast cancer of more advanced stages （Ⅲ and Ⅳ） were significantly higher than those in patients with stageⅠand stageⅡdiseases （P〈 0.05） ,however,there were no significantly differences between the values in patients with stageⅠand stageⅡdiseases （P〈 0.05） .Among the patients of the same stage,the serum expressions of NF-κB and CA15-3 in patients with axillary node metastasis were significantly higher than those in patients without metastasis （P〈 0.05） .Retrospective studies showed that the expressions in patients with recurrence one year after surgery were significantly higher than those in patients without recurrence （P〈 0.05） .Meanwhile,the serum expressions dropped markedly after successful surgery and the values in patients without recurrence one year after surgery were significantly lower than those before operation （P〈 0.05） .For diagnosis,combined determination of serum NF-κB and CA15-3 would yield a sensitivity of 90.6% .Conclusion NF-κB and CA15-3 were useful serum markers of breast cancer for clinical assessment.

关 键 词：乳腺癌 酶联免疫吸附试验 核因子-ΚB 糖类抗原15-3 转移 复发 

分 类 号：R737.9[医药卫生—肿瘤]