一株黑松-美味牛肝菌菌根辅助细菌的筛选及鉴定  

Isolation and Identification of a MHB Strain from the Rhizosphere Soil of Pinus thunbergi Inoculated with Boletus edulis

作　　者：盛江梅[1] 吴小芹[1] 侯亮亮[1] 应晨希[1] 

机构地区：[1]南京林业大学森林资源与环境学院,南京210037

出　　处：《应用与环境生物学报》2010年第5期701-704,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(No.30571471);"十一五"国家林业科技支撑计划专题(No.2006BAD08A1002);林业公益性行业科研专项(No.201004061)~~

摘　　要：为探讨菌根际有益微生物之间的作用关系及其互作对黑松(Pinus thunbergii)生长的影响,对分离自黑松-美味牛肝菌(Boletus edulis,Be)和黑松-黄色须腹菌(Rhizopogen luteous)菌根际土壤的81株细菌进行菌根辅助细菌(Mycorrhiza helper bacteria,MHB)的筛选.通过干皿对抗法筛选获得1株具MHB潜力的菌株HB12,HB12菌株对Be菌丝生长的平均增长率达34.4%.盆栽试验结果表明,单接Be处理的黑松根系菌根侵染率为36.8%,而双接种Be和HB12菌株处理的菌根侵染率为54.5%;双接种Be和HB12处理、单接Be处理和单接HB12处理较未接种处理的黑松苗高提高44%、36%和24.5%,地径提高27.7%、22.9%和26.5%.由此可确定菌株HB12为黑松-美味牛肝菌的菌根辅助细菌.经形态观察、Biolog系统鉴定及16S rRNA分子鉴定,初步确定菌株HB12为蜡状芽孢杆菌(Bacillus cereus).To investigate the effect of ectomycorrhiza co-inoculated with mycorrhiza helper bacteria （MHB） on the growth of pine seedlings,we screened MHB from 81 bacterial strains isolated from rhizosphere soil of Pinus thunbergi inoculated with Boletus edulis （Be） or Rhizipogen luteous,and we used the methods of dry-plate,liquid co-culture test in vitro and coinculation to evaluate the potential MHB strains.The dry-plate confrontation assay revealed that HB12 significantly promoted the in vitro hyphal growth of Be with an increasing rate of 34.4%.B.edulis significantly increased mycorrhizal formation in the presence of HB12 （54.5%） compared with the no-bacteria control （36.8%）.Compared with the blank control （-Be,-HB12）,the pine seedlings co-inoculated with Be and HB12 strain,Be alone （＋Be,-HB12）,and HB12 alone （＋HB12,-Be,） could significantly improve their mean height with increasing rates of 44%,36% and 24.5%,and their diameter with increasing rates of 27.7%,22.9% and 26.5%,respectively.The MHB stain was identified based on the analysis of morphological characters,Biolog system and 16S rRNA gene sequences homology.HB12 was identified as Bacillus cereus,of which 16S rRNA sequences have been registered at GenBank database under the accession number FJ040806.

关 键 词：黑松 美味牛肝菌 根际 菌根辅助细菌 蜡状芽孢杆菌 

分 类 号：S646.3[农业科学—蔬菜学]