一株兼性嗜冷小甲烷粒菌的生物学特性及系统发育分析  

Biological Characteristics and Phylogenetic Analysis of a Strain of Methanocorpusculum parvum

作　　者：袁敏[1,2] 张辉[1,2] 胡国全[1,2] 

机构地区：[1]农业部沼气科学研究所,成都610041 [2]农业部能源微生物与利用重点开放实验室,成都610041

出　　处：《应用与环境生物学报》2010年第5期705-709,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家高技术研究发展计划(863计划;No.2007AA100705);四川省重点科技攻关课题(No.2006Z07-004-02);科研院所技术开发专项资金(No.2009EG134272)~~

摘　　要：采用改进的亨盖特(Hungate)厌氧技术,从西藏林芝厌氧消化系统中分离到一株产甲烷菌菌株LZ-6.该菌株为革兰氏阴性,不运动,球形,直径约0.3～0.6μm.该菌株利用H2/CO2,微利用甲酸生长,不利用乙酸、甲醇、甲醇/H2、三甲胺、甲胺,最适生长pH为6.8～7.2,最适生长温度25℃,最适Na+浓度0.2mol/L.菌株LZ-6的16SrRNA基因序列与小甲烷粒菌(Methanocorpusculum parvum)相似性为99%.生理、形态结构特征等生物学特性研究表明此株产甲烷古菌为兼性嗜冷产甲烷古菌.A strain of methanogen LZ-6 was isolated from an anaerobic digester in Tibet,China by Hungate anaerobic technique.Of the strain,the cells were Gram negative,nonmotile,cocci of 0.3～0.6 μm in diameter.The catabolic substrates used included H 2 /CO 2 and formate,but no acetate,methanol,methanol/H 2 or methylated amines.Its growth was observed in a temperature range between 4 ℃ and 35 ℃ with an optimum at 25 ℃.The NaCl optimum for growth was 0.2 mol/L,and the fastest growth occurred within a pH range of 6.8～7.2.Strain LZ-6 was 99% similar in 16S rRNA gene sequence to Methanocorpusculum parvum.Its physiological,morphological and structural characteristics reveal that the strain is a eurypsychrophile methanogen.

关 键 词：小甲烷粒菌 产甲烷菌 16S RDNA 系统发育分析 

分 类 号：X172[环境科学与工程—环境科学]