神经营养因子3对急性肝损伤大鼠消化间期移行性运动复合波的影响  

Effects of neurotrophin 3 on interdigestive migrating motor complex in rats with acute liver injury

作　　者：刘梅[1] 许伟红[2] 段钟平[1] 郑素军[1] 陈煜[1] 刘旭华[1] 赵军[1] 丁美[1] 吕志武[2] 

机构地区：[1]首都医科大学附属北京佑安医院人工肝治疗培训中心,北京100069 [2]哈尔滨医科大学附属第一医院消化科,黑龙江哈尔滨150086

出　　处：《南方医科大学学报》2010年第9期2165-2168,共4页Journal of Southern Medical University

基　　金：北京市教育委员会科技发展计划项目(KM201010025024);国家自然科学基金资助项目(30800979;30800517);"艾滋病和病毒性肝炎等重大传染病防治"科技重大专项课题(2008ZX10002-005-3);北京市科技新星项目(2007B055);北京市自然科学基金资助项目(7102085)~~

摘　　要：目的观察神经营养因子3(NT-3)对急性肝损伤大鼠胃肠消化间期移行性运动复合波(migrating motorcomplex,MMC)的影响。方法选用纯种无特定病原体SD大鼠24只,等分为正常对照组和急性肝损伤组。正常对照组只给予等量生理盐水尾静脉注射;用D-氨基半乳糖诱导急性肝损伤大鼠模型、多道生理记录仪分别记录急性肝损伤组大鼠尾静脉注射NT-3前后胃肠消化间期MMC,并对大鼠胃肠消化间期MMC的各项指标进行分析。ELLISA检测正常对照组与急性肝损伤组大鼠血清NT-3浓度。急性肝损伤组大鼠尾静脉注射NT-3前后胃肠消化间期MMC,并对大鼠胃肠消化间期MMC的各项指标进行分析。ELLISA检测正常对照组与急性肝损伤组大鼠血清NT-3浓度。结果与急性肝损伤组大鼠尾静脉注射NT-3前相比较,注射NT-3后大鼠胃窦MMC周期变化无显著性差异(P>0.05),MMCⅠ相、MMCⅢ相及MMCⅣ相持续时间显著延长[(577.44±248.60)s vs(343.58±227.30)s,(80.94±21.15)s vs(24.76±7.41)s,(405.69±131.34)s vs(191.67±128.15)s,P<0.05],MMCⅡ相持续时间明显缩短[(883.94±488.50)s vs(1519.00±831.14)s,P<0.05];大鼠十二指肠MMC周期变化无显著性差异(P>0.05),MMCⅠ相、MMCⅢ相及MMCⅣ相持续时间显著延长[(557.63±335.14)s vs(309.46±220.22)s,(75.91±15.75)s vs(31.15±13.67)s,(423.38±135.22)s vs(209.77±123.83)s,P<0.05],MMCⅡ相持续时间明显缩短[(748.81±579.69)s vs(1535.86±930.50)s,P<0.05];大鼠空肠MMC周期及MMCⅠ相变化无显著性差异(P>0.05),MMCⅢ相及MMCⅣ相持续时间显著延长[(86.58±23.40)s vs(31.41±16.09)s,(385.18±110.02)svs(220.59±159.30)s,P<0.05],MMCⅡ相持续时间明显缩短[(876.89±652.01)s vs(1870.89±1010.35)s,P<0.05]。急性肝损伤组大鼠血清NT-3浓度明显高于正常对照组大鼠[(0.75±0.49)ng/ml vs(0.27±0.01)ng/ml,P<0.05],差异有统计学意义。结论 NT-3可增强急性肝损伤大鼠胃肠动力。Objective To observe the effects of neurotrophin 3（NT-3）on interdigestive migrating motor complex（MMC） in rats with D-galactosamine induced acute liverinjury.Methods Twenty-four specific pathogen-free purebred rats were equally randomized into control and acute liverinjury groups.The control group was injected with equal volume of normal saline via tail vein.Acute liver injury model of the rats was induced by D-galactosamine injection via the tail vein in the acute liverinjury group.And the indexes of interdigestive MMC before and after NT-3 injection were recorded by a polygraph and analyzed in model group.The serum NT-3 concentration was assayed in the two groups.Results There were no significant changes of gastrointestinal MMC cycle and jejunal phase I MMC after NT-3 injection.Compared with the acute liverinjury rats before NT-3 injection,the antral phases I,III and IV MMC were significantly prolonged [（577.44±248.60）s vs（343.58±227.30）s,（80.94±21.15）s vs（24.76±7.41）s,（405.69±131.34）s vs（191.67±128.15）s,P〈0.05] and the phase Ⅱ MMC was shortened [（883.94± 488.50）s vs（1519.00±831.14）s,P〈0.05] in the acute liver injury group.The duodenal phasesⅠ,Ⅲ and Ⅳ MMC were significantly prolonged [（557.63±335.14）s vs（309.46± 220.22）s,（75.91 ±15.75）s vs（31.15 ±13.67）s,（423.38 ± 135.22）s vs（209.77±123.83）s,P〈0.05] and MMCⅡphasewas shortened [（748.81±579.69）s vs（1535.86±930.50）s,P〈0.05]in the acute liver injury rats.In addition,the jejunal MMC Ⅲ and MMCⅣ phase was significantly prolonged [（86.58±23.40）s vs（31.41±16.09）s,（385.18±110.02）s vs（220.59± 159.30）s,P〈0.05] and phaseⅡMMC was shortened [（876.89±652.01）s vs（1870.89±1010.35）s,P〈0.05]in the acute liver injury rats.The serum NT-3 level was significantly higher in model group than in control group.Conclusion NT-3 could enhance the gastrointestinal motility in acute liverinjury rats.

关 键 词：神经营养因子3 肝损伤 急性 消化间期移行性运动复合波 

分 类 号：R575[医药卫生—消化系统]