克癌新抗肿瘤作用机制研究  

Study on antitumor Mechanism of Keaixin

作　　者：白映涛[1] 任美萍[2] 李蓉[2] 刘明华[2] 肖顺汉[2] 李茂[2] 

机构地区：[1]中江县人民医院,四川中江618100 [2]泸州医学院药理毒理研究室,四川德阳618100

出　　处：《医学信息（中旬刊）》2010年第11期3125-3127,共3页Medical Information Operations Sciences Fascicule

基　　金：四川省教育厅重点项目(No2005A076)

摘　　要：目的:研究克癌新对细胞增殖、凋亡以及对荷瘤小鼠细胞因子水平的影响。方法:人肝癌细胞株SMMC-7721、人乳腺癌细胞株MDA231和人肺癌细胞株A549为模型,采用CCK-8试剂盒(cellcountingkit-8)检测对克癌新对细胞增殖的抑制作用;透射电镜观察对人肝癌细胞株SMMC-7721凋亡的超微结构变化;建立小鼠肉瘤S180和小鼠肝癌H22移植性肿瘤模型,采用Elisa法测定细胞因子白介素-2(IL-2)、白介素-12(IL-12)的水平。结果:克癌新对SMMC-7721、MDA231和A549细胞的增殖无明显影响;SMMC-7721细胞透射电镜可见核固缩、碎裂和凋亡小体等凋亡形态学改变;克癌新在150、300mg.Kg-1时对小鼠肉瘤S180的生长抑制率分别为38.52%、47.88%,对肝癌H22生长抑制率分别为32.17%、40.18%,且能提高荷瘤小鼠的脾指数和胸腺指数,克癌新能升高荷瘤小鼠细胞因子IL-2、IL-12的水平。结论:克癌新对细胞增殖无明显影响,可诱导细胞凋亡。Objective ：To study the effects of Keaixin on the proliferation, apoptosis and level of eytokine in tumor- beating mice. Methods：The cytotoxicity of Keaixin on human hepatoma carcinoma cell line SMMC -7221, human breast carcinoma MDA231 and lung adenocarcinoma A549 cell lines were determined by cell counting kit - 8 （ CCK -8） assay. The morphological changes of the apoptusis cells were observed by transmission electron microscope. The inhibitory effect of Keaixin on tumor growth was observed by the models of implanted sarcoma 180 （S180） and hepatoma 22 （H22） in mice; The levels of IL - 2 and IL - 12 in tumor - beating mice was investigated by Elisa assay. Results：Keaixin have no effect on the proliferation of SMMC -7721 ,MDA231 and A549 cells. SMMC -7221 cells apoposis was morphologically observed after which were induced by Keaixin. At dosage of 150 and 300mg· Kg -1 ,the inhibitory rate of Keaixin is 38.52% and 47.88% for S180,32. 17% and 40.18% for H22,respectively. Keaixin can increase spleen index and thymus gland index,and enhance the expression levels of IL -2and IL - 12. Conclu- sions ： Keaixin have no effect on the proliferation of cells. Keaixin can induce their apoptosis. Keaixin exhibited significantly anti - tumor effect in vivo and the mechanism may be related to enhancing the level of cvtokine.

关 键 词：克癌新 凋亡 细胞因子 

分 类 号：R735.7[医药卫生—肿瘤]