香兰素衍生物VND3207对小鼠骨髓细胞遗传损伤的防护效应研究  

Radiological protection effect of vanillin derivative VND3207 against radiation-induced cytogenetic damage in mouse bone marrow cells

作　　者：汪传高[1] 汪黎[1] 周平坤[1] 王仲文[1] 胡永哲[1] 靳海明[1] 张学清[1] 陈英[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《中华放射医学与防护杂志》2010年第5期558-560,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金杰出青年基金（C30825011）

摘　　要：目的 研究香兰素衍生物4-羟基-3,5-二甲氧基苯甲醛（VND3207）对γ射线整体照射小鼠骨髓细胞遗传损伤的防护作用.方法 BALB/c小鼠按10、50和100 mg/kg不同剂量灌胃给药,1次/d,连续5 d,最后一次给药后2 h,2 Gy60Co γ射线照射,照射后48 h观察小鼠骨髓嗜多染红细胞微核、染色体畸变和骨髓有丝分裂指数的改变.结果 不同剂量（10、50和100 mg/kg）的VND3207均能有效地降低小鼠骨髓嗜多染红细胞微核率（t=2.40～4.26,P＜0.05）和染色体畸变率（t=2.36～3.52,P＜0.05）,同时提高骨髓有丝分裂指数.药物保护效果与给药剂量呈依赖关系,其中100 mg/kg剂量组的微核率和染色体畸变率与单纯照射组相比,分别降低了65%和50%.结论 VND3207对60Co γ射线诱发的小鼠骨髓辐射损伤有良好的保护作用.Objective To study the protection of vanillin derivative VND3207 on the cytogenetic damage of mouse bone marrow cell induced by ionizing radiation.Methods BALB/c mice were randomly divided into five groups：normal control group,2 Gy dose irradiation group,and three groups of 2 Gy irradiaiton with VND3207 protection at doses of 10,50 and 100 mg/kg,respectively.VND3207 was given by intragastric administration once a day for five days.Two hours after the last drug administration,the mice were irradiated with 2 Gy γ-rays.The changes of polychromatophilic erythroblasts micronuclei （MN）,chromosome aberration （CA） and mitosis index （MI） of mouse bone marrow cells were observed at 24 and 48 h after irradiation.Results Under the protection of VND3207 at the dosages 10,50,100 mg/kg,the yields of poly-chromatophilic erythroblasts MN and CA of bone marrow cells were significantly decreased（t = 2.36-4.26,P ＜ 0.05）,and the marrow cells MI remained much higher level compared with the irradiated mice without drug protection （t = 2.58,2.01,P ＜ 0.05）.The radiological protection effect was drug dose-dependent,and the administration of VND3207 at the dosage of 100 mg/kg resulted in reduction by 50% and 65% in the yields of MN and CA,respectively.Conclusions VND3207 had a good protection effect of on γ-ray induced cytogentic damage of mouse bone marrow cells.

关 键 词：香兰素衍生物 Γ射线 嗜多染红细胞微核 染色体畸变 有丝分裂指数 辐射防护剂 

分 类 号：R285.5[医药卫生—中药学]