18氟-氟代脱氧葡萄糖对Lewis肺癌细胞增殖的影响  

Effect of 18F-2-deoxy-2-fluoro-d-glucose on proliferation of Lewis lung cancer cell

作　　者：王明明[1] 司原[1] 陶新全[1] 张伍魁[1] 谢强[2] 谢吉奎[2] 

机构地区：[1]安徽医科大学核医学教研室,合肥230032 [2]安徽省立医院PET-CT中心

出　　处：《中华放射医学与防护杂志》2010年第5期568-571,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：安徽省教育厅自然科学基金（2006KJ3123）

摘　　要：目的 研究不同浓度18氟-氟代脱氧葡萄糖（18F-FDG）对Lewis肺癌细胞增殖的影响,探讨其作用机制.方法 以0、0.37、1.85、3.70和7.4（×106 Bq/ml）18F-FDG处理细胞24 h,用倒置显微镜与电子显微镜观察细胞形态学改变,流式细胞术检测细胞凋亡与细胞周期时相分布,3H-TdR掺入法检测细胞DNA合成,比色法检测细胞脂质过氧化水平,免疫组织化学法检测细胞Bcl-2和Bax蛋白的表达.结果 细胞的累积吸收剂量分别为0、0.1l、0.55、1.10与2.20 Gy.受照细胞出现凋亡形态学改变,在0～2.20 Gy剂量范围内,细胞凋亡率由（4.05±0.01）%增大为（25.6±0.28）%（t=188,P＜0.01）,3H-TdR掺入率从100%下降为（22.0±0.51）%（t=27.6,P＜0.05）,细胞内丙二醛（MDA）含量由（0.08±0.03）上升到（0.67±0.12）μmol/L（t=11.7,P＜0.01）.Bcl-2蛋白表达降低,Bax蛋白表达增高,细胞周期发生G2/M期阻滞.结论 18F-FDG能诱导Lewis肺癌细胞凋亡,抑制细胞增殖.Objective To investigate the influence of 18F-FDG on the proliferation of Lewis lung cancer cell line,and to elucidate its possible mechanism.Methods Morphological changes of cells after culture for 24 h at different concentrations of 0,0.37,1.85,3.70 and 7.4 （×106） Bq/ml of 18F-FDG were observed by using inverted microscopy and electron microscopy.The apoptosis and phase distribution of cell cycle of irradiated cells were analyzed with flow cytometry.DNA synthesis of irradiated cells was assayed by 3H-TdR incorporation.Lipid peroxidation was measured by chromometry and expression of Bcl-2 and Bax protein was measured by immunohistochemical technique.Results Exposed to （0-7.40） × 106Bq/ml of 18F-FDG for 24 h,the cumulative absorbed doses delivered to cells in five groups were 0,0.11,0.55,1.10 and 2.20 Gy,respectively.Irradiated cells showed morphological changes of apoptosis.The apoptosis rate of irradiated cells was increased from （4.05 ± 0.01）% to （25.6 ± 0.28） % （t = 188,P＜0.01）.3H-TdR incorporation rate was decreased from 100% to（22.0 ± 0.51）% （t =27.6,P ＜0.05）.The levels of M DA in cells were augmented from （0.08 ± 0.03） to （0.67 ± 0.12） μmol/L （t =11.7,P ＜ 0.01）.Cell cycle arrest was found in G2/M phase with the increasing doses from 0 to 2.20 Gy.The expression of Bcl-2 protein was decreased while that of Bax protein increased.Conclusions 18F-FDG could induce the apoptosis of cells and inhibit the proliferation of cells.

关 键 词：18氟-氟代脱氧葡萄糖 细胞凋亡 BCL-2蛋白 BAX蛋白 

分 类 号：R734.2[医药卫生—肿瘤]