大鼠视上核、室旁核肠三叶因子表达与实验性胃溃疡的关系  

Expression of intestinal trefoil factor on supraoptic nucleus and paraventricular nucleus in rat hypothalamus and its relationship with experimental gastric ulcer

作　　者：吴靖芳[1] 史宝林[2] 张静[1] 郑慧娥[1] 张耕[1] 任君旭[1] 

机构地区：[1]河北北方学院组织学与胚胎学教研室,河北张家口075000 [2]河北北方学院公共卫生教研室,河北张家口075000

出　　处：《解剖学报》2010年第5期660-665,共6页Acta Anatomica Sinica

基　　金：河北省自然科学基金(C2007000906);河北省卫生厅重大项目基金(07138)资助

摘　　要：目的探讨大鼠实验性胃溃疡自愈期间肠三叶因子(ITF)在下丘脑视上核(SON)、室旁核(PVN)的表达及其与实验性胃溃疡愈合的关系。方法以免疫组织化学染色、酶联免疫吸附实验分别检测溃疡组(42只)和正常组(6只)大鼠下丘脑和血清及ITF的表达及含量变化,RT-PCR检测ITFmRNA的转录情况。结果 ITF免疫反应阳性物质主要位于视上核和室旁核大细胞部。溃疡1d视上核和室旁核ITF积分吸光度略增高,2d、4d和6d逐渐升高,6d达高峰(P<0.01),10～23d均维持在较高水平(P<0.05)。血清ITF的变化与免疫组织化学法结果相似;溃疡组ITF/3-磷酸甘油醛脱氢酶(GAPDH)吸光度比值在溃疡2～23d均高于正常组(P<0.01,或P<0.05)。结论胃溃疡自愈期间可能通过下丘脑和血清ITF的高表达参与溃疡愈合的调节。Objective The purpose of this study is to explore the expression of intestinal trefoil factor （ITF） on supraoptic nucleus（SON） and paraventricular nucleus（PVN） in rat hypothalamus and its relationship with experimental gastric ulcer. Methods The changes of ITF in hypothalamus and blood serum in gastric ulcer group（GU） and normal group（N） rats were detected by means of immunohistochemical staining method and enzyme-linked immunosorbent assay （ELISA）. The transcription of ITF mRNA was detected by RT-PCR. Results ITF immunoreactive cells were mainly located in SON and magnocellular region of PVN （mPVN）, The integral absorbance（IA） was obviously increased from the day 2 to 6 after gastric ulcer（ P 〈 0.01 ） , and reached a highest expression at the 6th day. then gradually decreased from 10 days to 23 days,but remained on a high level compared with the normal group（ P 〈 0.05 ）. The change of ITF peptide in serum was similar to the immunohistochemical results. The absorbance of ITF/glyceral dehyde 3-phosphate dehydrogenase （GAPDH） was obviously higher in gastric ulcer group than that of the normal group from 2 days to 23 days（P 〈0. 01 or P 〈 0. 05）. Conclusion The high expression of ITF in rat hypothalamus and blood serum may participate in the regulation of self-healing in gastric ulcer.

关 键 词：肠三叶因子 视上核 室旁核 实验性胃溃疡 反转录-聚合酶链式反应 大鼠 

分 类 号：R333.3[医药卫生—人体生理学]