卵巢上皮恶性肿瘤侵袭转移相关miRNA的筛选与鉴定  

Screening and identification of potential miRNA involved in ovarian cancer invasion and metastasis

作　　者：梁山辉 李俊 Mafia Al-beit 张进 马端 鹿欣 

机构地区：[1]复旦大学附属妇产科医院妇科,上海200011

出　　处：《中华肿瘤杂志》2010年第9期650-654,共5页Chinese Journal of Oncology

摘　　要：目的 探索与卵巢上皮恶性肿瘤侵袭转移可能相关的miRNA.方法 采用miRNA芯片,筛选SKOV-3ip和SKOV-3细胞差异表达miRNA.利用生物信息学软件TargetScan、MicroCosm、PicTar和GO,预测差异表达miRNA的靶基因及其功能.采用实时逆转录聚合酶链反应（real-time RT-PCR）技术,验证与卵巢癌侵袭转移可能相关的5种miRNA（let-7a、let-7e、let-7f、miR-22和miR-886-5p）在SKOV-3ip和SKOV-3细胞的表达.同时,检测这5种miRNA在另外一组侵袭转移能力不同的卵巢癌细胞株HO8910和HO-8910PM中的表达,并进行统计学分析.结果 基因芯片筛选显示,42种miRNA在SKOV-3ip和SKOV-3细胞株表达差异明显.进一步分析显示,let-7a、let-7e、let-7f、miR-22和miR-886-5p等5种miRNA可能与卵巢癌的侵袭转移密切相关.real-time RT-PCR结果证实,let-7f和miR-22在两组侵袭转移能力不同的卵巢癌细胞（SKOV-3和SKOV-3ip细胞、HO-8910和HO-8910PM细胞）表达差异有统计学意义（均P＜0.05）.结论 let-7f和miR-22在侵袭转移能力强的卵巢癌细胞中低表达,可能具有抑癌基因的作用.Objective To identify potential miRNA involved in epithelial ovarian cancer （EOC）invasion and metastasis. Methods miRNA microarray was applied to compare the miRNA expression profile between SKOV-3ip and SKOV-3 cells. Bioinformatics programs （TargetScan, MicroCosm, PicTar and GO） were used to analyze the miRNA and their potential target genes. Real-time RT-PCR was used to confirm the results of microarray and for expanding detection in another paired EOC cell lines （HO-8910 and HO-8910PM）. Results Totally, expressions of 42 miRNA were found significantly different between SKOV-3ip and SKOV-3 cells. Among them, 10 miRNA were down-regulated, including let-7a, let-7f, miR22 and miR-886-5p; while 32 were up-regulated, for example, let-7e and miR-519e. Bioinformatic analysis indicated that let-7a, let-7e, let-7f, miR-22 and miR-886-5p may be involved in cancer invasion and metastasis. Meanwhile, real-time RT-PCR confirmation and statistic analysis showed that let-7f and miR-22expressions were significantly different between ovarian cancer cell lines with various invasive and metastatic capacity （P 〈 0.05）. Conclusion The expression of let-7f and miR-22 is low in ovarian cancer cells with high invasive and metastatic capacity. It suggests that they are potential tumor suppressor genes. Further research on their role and mechanism is needed.

关 键 词：卵巢肿瘤 MIRNA 肿瘤侵袭 肿瘤转移 基因芯片 实时逆转录聚合酶链反应 

分 类 号：R[医药卫生]