抑癌基因Runx3启动子甲基化与喉癌发生和转移的关系  

Relationship between hypermethylation of Runx3 gene and the development and metastasis of laryngeal Carcinoma

作　　者：汤靓[1] 白伟良[1] 季文樾[1] 高红[2] 刘姣[1] 

机构地区：[1]中国医科大学附属盛京医院耳鼻咽喉科,沈阳110004 [2]中国医科大学附属盛京医院卫生部小儿先天畸形重点实验室,沈阳110004

出　　处：《临床耳鼻咽喉头颈外科杂志》2010年第18期834-837,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：辽宁省博士启动基金资助(No:20051038);沈阳市社会发展基金资助(No:041101)

摘　　要：目的:探讨Runx3基因启动子甲基化与喉癌发生发展过程的关系。方法:采用RT-PCR检测40例喉癌组织及肿瘤周围黏膜组织中Runx3 mRNA的表达,同时采用甲基化特异性PCR(MSP)方法检测Runx3基因启动子甲基化情况。结果:40例喉癌组织标本中Runx3基因的表达(1.62±1.01)较肿瘤周围组织中表达(5.66±2.07)明显下调,差异有统计学意义(P<0.01)。正常黏膜组织中未发现有Runx3基因启动子的甲基化,40例喉癌组织中有38例检测到Runx3基因启动子的甲基化,喉癌组织Runx3基因启动子甲基化率显著增高(P<0.01)。喉癌标本中Runx3 mRNA的表达与其病理临床特征密切相关,在低分化和有淋巴结转移喉癌组织标本中Runx3 mRNA的表达显著下调(P<0.01)。结论:Runx3基因启动子甲基化是导致Runx3基因失活的主要原因之一并且与喉癌的发生、发展密切相关。Objective：To explore the relationships between hypermethylation of human runt-related transcription factor 3（Runx3）gene promoter and laryngeal squamous cell cancer.Method：Promoter hypermethylation and mRNA expression were detected by methylation-specific PCR and RT-PCR.Result：The expression of Runx3 gene mRNA detected in laryngeal carcinoma（1.62±1.01）was lower than that in adjacent tissues samples（5.66±2.07） （t=10.72,P0.01）.No methylation of Runx3 promoter was found in adjacent tissues samples.But hypermeth-ylation was found in 95.0% （38/40）of the laryngeal carcinoma specimens.The rate of methylation of Runx3 pomoter in laryngeal carcinoma was higher than that in adjacent tissues（P0.01）.The Runx3 mRNA were down-regulated in lymphnode metastasis or poorly differentiated groups,but the Runx3 promoter methylation were detected in those groups markedly.Conclusion：Hypermethylation of Runx3 promoter is one of the inactivation reseasons in laryngeal squamous cell carcinoma,and the decreasing of Runx3 mRNA expression may be related to lymph node metastasis and development of laryngeal squamous cell carcinoma.

关 键 词：喉肿瘤 RUNX3基因 DNA甲基化 淋巴结转移 

分 类 号：R739.65[医药卫生—肿瘤]