大豆球蛋白基因表达载体的构建及对桑树的遗传转化  

Construction of Expression Vector for Glycinin Gene and Its Transformation to Morus alba

作　　者：谈建中[1] 楼程富[1] 钟名其[1] 平野久[2] 

机构地区：[1]浙江农业大学蚕学系 [2]日本横滨市立大学木原生物学研究所

出　　处：《蚕业科学》1999年第1期5-10,共6页ACTA SERICOLOGICA SINICA

基　　金：国家自然科学基金

摘　　要：为探讨植物转基因技术在桑树品质改良上的应用途径，构建了含大豆球蛋白亚基（ＡｌａＢｌｂ）基因的表达载体，将其转入根癌农杆菌后，用农杆菌介导法转化桑树叶盘与茎尖，经卡那霉素抗性筛选培养，再生形成了不定芽及试管幼苗，ＰＣＲ检测证实ＡｌａＢｌｂ亚基基因已转入桑树。In order to investigate the possibility of improving mulberry quality by transgenic techniques,expression vector carrying glycinin subunit AlaBlb gene was constructed and transferred into Agrobacterium tumefaciens. And then,leaf disk and stem apex of mulberry were used as receptor materials of genetic transformation induced by Agrobacterium. The regeneration plantlets with kanamycin resistance were obtained from these materials.Results by PCR indicated that AlaBlb subunit gene was transformed into mulberry plant.

关 键 词：桑树 转基因 大豆球蛋白 根癌农杆菌 

分 类 号：S888.2[农业科学—特种经济动物饲养] S188[农业科学—畜牧兽医]