HPLC法测定蛹虫草子实体及其培养基的虫草素含量  

Determination of Cordycepin in Cordyceps and its Culture Medium by HPLC

作　　者：黄国鑫[1] 任薇[1] 吕在坤 严钦惠 苏子仁[1] 

机构地区：[1]广州中医药大学,广东广州510006 [2]广东雅娜集团有限公司,广东广州510400

出　　处：《亚太传统医药》2010年第10期36-38,共3页Asia-Pacific Traditional Medicine

摘　　要：目的:建立蛹虫草子实体及其培养基中虫草素(Cordycepin)含量的测定方法。方法:采用高效液相色谱(HPLC)法,选用YMC-PackC18色谱柱(250×4.6mm,5μm),流动相为甲醇-水(20:80),流速为1mL/min,检测波长为260nm,柱温为30℃,分析时间为20min。结果:蛹虫草子实体中虫草素含量为0.503mg/g,平均加样回收率(n=9)为100.8%(RSD=1.33%);蛹虫草培养基中虫草素含量为0.118mg/g,平均加样回收率(n=9)为101.7%(RSD=1.82%)。结论:本方法分离度好、精密度高、专属性强、灵敏度高,可用于蛹虫草子实体及其培养基的质量评价。Objective：A new HPLC method was developed to determine the content of the Cordycepin in fruitbodies as well as Culture medium.Methods：HPLC was performed on a YMC-Pack C18 reversed-phase column（250×4.6mm,5μm） .All of the reference substances and sample were separated with the mobile phase of methanol：water（20：80） under isocratic elution for 20min,flow rate was 1.0mL/min,the detection wavelength was 260nm,and the column temperature was 30℃.Results：The content of the Cordycepin in the fruitbody were 0.503mg/g and 0.118mg/g in the Culture medium.The average recovery rate were about 101.1%（n=9,RSD=1.33%） in the fruitbody and 101.7%（n=9,RSD=1.82%） in the Culture medium.Conclusion：This developed method,which is simple and with high sensitivity as well as selectivity,can be used for quality evaluation of the Cordyceps.

关 键 词：高效液相色谱法 蛹虫草 虫草素 

分 类 号：R284[医药卫生—中药学]