大米草多糖的提取及其单糖组成的GC-MS分析  

Extraction of polysaccharides from Spartina anglica and analysis of the monosaccharide composition by gas chromatography-mass spectrometry

作　　者：徐年军[1] 何艳丽[1] 武敏[1] 严小军[1] 

机构地区：[1]宁波大学生命科学与生物工程学院,教育部应用海洋生物技术重点实验室,浙江宁波315211

出　　处：《海洋科学》2010年第9期15-18,共4页Marine Sciences

基　　金：浙江省卫生厅项目(2005B149);浙江省自然科学基金项目(Y507263);浙江省钱江人才计划项目(2007R10038);宁波市自然科学基金项目(2007A610071)

摘　　要：分析了海洋滩涂生物大米草(Spartina anglica)中的多糖成分和单糖组成,为这种生物资源的开发提供科学依据。利用水提醇沉、Sevag法脱蛋白得到纯大米草多糖,经三氟乙酸降解、糖腈乙酸酯衍生,最后用气相色谱-质谱联用法(GC-MS)分析大米草多糖中的单糖组成。结果表明大米草单糖组成为鼠李糖、核糖、阿拉伯糖、木糖、甘露糖、葡萄糖、半乳糖,其质量分数分别是:4.13%,3.02%,11.94%,8.89%,6.82%,27.18%,38.00%。大米草多糖是一种可利用的食品资源,其主要成分半乳糖、葡萄糖都是营养价值很高的单糖,大米草多糖可深入研究开发其生物活性用作保健食品。To study the polysaccharides and analysis their monosaccharide compositions of the Spartina anglica,purified polysaccharides were obtained by hot water extraction,ethanol precipitation,and de-protein by Sevag method.The polysaccharides were hydrolyzed to monosaccharides by 2 mol/mL trifluoroacetic acid in 120℃ oil-bath for 1h.The derived monosaccharides were analyzed by gas chromatography-mass spectrometry（GC-MS）.The S.anglica polysaccharides consisted of rhamnose,ribose,arabinose,xylopyranose,mannose,glucose,lactose,with their relative percentages being 4.13%,3.02%,11.94%,8.89%,6.82%,27.18%,and 38.00%,respectively.It is predicted that the polysaccharides of the S.anglca are a potential food resource,with the main monosaccharides,i.e.lactose and glucose,being of very high nutritional value.Further study for their applications in functional foods is warranted.

关 键 词：大米草(Spartina anglica) 多糖 单糖组成 GC-MS分析 

分 类 号：Q539[生物学—生物化学]