原生质体诱变选育ε-聚赖氨酸高产菌株  

Microbial Breeding of High ε-polylysine-producing Strains by Diethyl Sulfate Protoplast Mutagenesis

作　　者：田丰伟[1] 程传荣[1] 袁维涵[1] 赵鑫[1] 赵建新[1] 张灏[1] 陈卫[1] 

机构地区：[1]江南大学食品学院食品科学与技术国家重点实验室,江苏无锡214122

出　　处：《微生物学通报》2010年第10期1457-1461,共5页Microbiology China

基　　金：国家863计划项目(No.2007AA10Z353);国家"十一五"科技支撑计划项目(No.2009BADB9B05);国家自然科学基金项目(No.20836003)

摘　　要：以白色链霉菌UN2-71为出发菌株,对其原生质体进行硫酸二乙酯(DES)诱变,选育ε-聚赖氨酸高产菌株。经过试管初筛和摇瓶复筛,得到1株稳定性好的菌株D3-32,摇瓶产量达到1.56g/L,比出发菌株提高49.43%。采用2.3L发酵罐进行发酵试验,控制pH分两阶段培养后,ε-聚赖氨酸最高产量达到4.59g/L,比出发菌株提高了2.65倍。Streptomyces albulus UN2-71 was selected as original strain and its protoplast was treated by diethyl sulfate （DES） to obtain high ε-polylysine producing mutants.Through primary screening and confirmed screening with shake flask cultures,one strain designated as D3-32 with a good genetic stability has been acquired,whose production in the shake flask reached 1.56 g/L,which was about 49.43% higher than that of original strain.In the fermentation test with 2.3 L fermentor,after a pH-controlled two-phase fermentation,the highest production of ε-polylysine reached 4.59 g/L,which was increased by 2.65 times compared to that of the original strain.

关 键 词：Ε-聚赖氨酸 白色链霉菌 原生质体 硫酸二乙酯 诱变 

分 类 号：Q93[生物学—微生物学]