高频重复经颅磁刺激对大鼠神经元及血脑屏障超微结构的影响  

Effects of High-frequency Repetitive Transcranial Magnetic Stimulation on Ultrastructure of Neurons and Blood-brain Barrier in Rats

作　　者：方征宇[1] 岳翔[1] 黄晓琳[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院康复医学科,武汉430030

出　　处：《中国康复》2010年第5期327-329,共3页Chinese Journal of Rehabilitation

基　　金：国家自然科学基金资助项目(No:30801220);华中科技大学同济医学院附属同济医院科研基金(2008)

摘　　要：目的:研究高频重复经颅磁刺激(rTMS)对大鼠神经元及血脑屏障超微结构的影响。方法:健康成年SD大鼠14只,随机分为对照组和rTMS组各7只,rTMS组以20 Hz、80%最大输出强度行rTMS,鼠脑中线旁左眼后眦与左耳前缘连线的中点接受最强磁刺激,每天1次,15 min。连续2周后取脑,制作超薄切片,在电子显微镜下观察神经元及血脑屏障的超微结构。结果:在刺激过程中,rTMS组未出现肢体强直、阵挛等异常活动。扫描电子显微镜下发现,rTMS组与对照组比较,神经元,核内染色质聚集边集,粗面内质网附着的核糖体数量和线粒体减少,部分线粒体水肿;毛细血管内皮细胞、基膜及周细胞出现明显水肿,部分神经元及毛细血管内皮细胞失去正常形态和结构。结论:长期高频rTMS可一定程度破坏大鼠神经元细胞器及血脑屏障。提示在将高频rTMS应用于大脑时应谨慎。Objective： To observe the effects of high-frequency repetitive transcranial magnetic stimulation（rTMS） on ultrastructure of neurons and blood-brain barrier in rats.Methods： Fourteen Sprague Dawley rats were randomly divided into two groups： control group and magnetic stimulation group.The animals in magnetic stimulation group were subjected to 20 Hz rTMS for 15 min per day at the intensity of 80% maximum output of stimulator for 14 days.Left motor cortex was selected as stimulation locus.The brains were removed,and the ultrathin sections were made.The changes of neurons and blood-brain barrier ultrastructure were observed under the electron microscopy.Results： During stimulation process,no limb rigidity and clonic seizure occurred.Under the scanning electron microscopy,neurons in rTMS group showed morphological changes： margination of chromatin,reduced number of mitochondria,mitochondria swelling.Blood brain barrier in rTMS group showed： swelling of endothelial cells,basement membrane and pericytes.Some neurons and blood brain barrier lost normal structure and organization.Conclusion： Long-term high-frequency transcranial magnetic stimulation may to some extent damage organelles in neurons and reduce the blood-brain barrier permeability.

关 键 词：磁刺激 神经元 血脑屏障 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]