人肝细胞系5-羟色胺自分泌系统的研究  

Study of autocrine loop of 5-HT in human liver cell lines

作　　者：李晓晴[1] 杨仙珊[1] 赵鹏[1] 丁美[1] 赵军[1] 段钟平[1] 张晶[1] 

机构地区：[1]首都医科大学附属北京佑安医院人工肝中心,100069

出　　处：《肝脏》2010年第4期269-271,共3页Chinese Hepatology

摘　　要：目的通过对人肝癌细胞系和人正常肝细胞系的研究,探讨肝细胞是否具有合成5-HT的功能。方法以人肝癌细胞系HepG2和人肝细胞系7702为研究对象,采用ELISA方法检测细胞培养上清液中的5-HT水平,采用荧光定量PCR法检测细胞表达5-HT合成的关键酶及5-HT受体。结果两种细胞系细胞上清液均可检测到5-HT;均可表达多种类型的5-HT受体,均表达合成5-HT的关键酶色氨酸羟化酶(TPH)及运载体(SERT)。结论人肝癌细胞系HepG2和人肝细胞系7702均表达合成5-HT的关键酶和受体,说明肝细胞具有5-HT自分泌系统,5-HT可能以自分泌或者旁分泌的形式发挥作用。Objective To identify whether liver cells including human hepatocarcinoma cell line and human normal liver cell line can synthesize 5-HT.Methods The level of 5-HT in cell supernatant was detected by ELISA.The expression of 5-HT receptors and key enzymes which take part in synthesis or metabolism of 5-HT were determined by Real Time PCR.Results 5-HT could be detected in the supernatant of the two cell lines.The two cell lines could expression different types of 5-HT receptors and key enzymes.Conclusion The human liver cells possess autocrine loop of 5-HT,it may work by the form of autocirne or paracrine.

关 键 词：人肝癌细胞系 人正常肝细胞系 5羟色胺 自分泌 

分 类 号：R735.7[医药卫生—肿瘤]