大鼠实验性牙移动中P2X3受体在牙周膜的表达变化研究  

Expression of P2X3 receptors in rat periodontal ligament during experimental tooth movement

作　　者：卢云[1] 华小川[2] 常新[1] 贵林[1] 王晟[3] 赖文莉[3] 

机构地区：[1]大连医科大学口腔医学院, 116044 [2]云南省第二人民医院门腔正畸科 [3]四川大学华西口腔医院正畸科,610041

出　　处：《中华口腔正畸学杂志》2010年第3期149-152,共4页Chinese Journal of Orthodontics

基　　金：基金项目：国家自然科学基金资助项目（30471912）

摘　　要：目的 探讨大鼠实验性牙移动中P2X3受体在牙周膜的表达变化,初步了解P2X3受体与正畸牙移动疼痛信息传递的关系.方法 54只雄性SD大鼠（体重200～250 g）随机分为空白组（5只）、对照组（14只）和实验组（35只）.选择左侧上颌第一磨牙作为观察对象,制备大鼠正畸牙移动不同时间点牙周膜组织切片.结果 正畸牙移动加力后,牙周膜压力侧和张力侧P2X3受体免疫阳性表达增加,呈短时上调规律,两侧相同时间点进行两两比较,结果无统计学意义.结论 正畸牙移动疼痛信息传递过程中P2X3受体在牙周膜压力侧与张力侧呈一过性上调规律,推测P2X3受体与牙移动伤害表达密切相关,但其作用机制还有待进一步研究.Objective To investigate the P2X3 receptors changes in rat periodontal ligament during experimental tooth movement（ETM）, and find its possible effect during ETM. Methods 54male SD rats （200～250 g） were selected in this study. There were 7 experimental groups （35 rats）and 2 control groups [Group A： intact rat （5 rats）; Group B： sham rat （14 rats）]. Biopsies of periodontal ligament in the left maxillary first molar region was obtained at different time points to carry out immunohistochemical study. Results Immunohistology was used to reveal the distribution of P2X3 receptors in periodontal ligament. Predominant up regulation of P2X3 receptors immunoactivity was found in periodontal ligament from 4h to 7d. No significant difference was found in immunoactivity of P2X3 receptors between the pressure and tensile sides at the same time.Conclusions P2X3 receptors have a rhythm change in rat periodontal ligament as a result of ETM.

关 键 词：实验性牙移动 疼痛 P2X3受体 

分 类 号：R783.5[医药卫生—口腔医学]