绒癌中Fas/FasL的表达及其介导的细胞凋亡的意义  

Significance of Fas/FasL expression and its apoptosis mechanism in the choriocarcinoma cell lines

作　　者：翟凌[1] 李哲田[2] 汪永胜[3] 卢美松[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院妇产科,黑龙江哈尔滨150001 [2]内蒙古鄂尔多斯市中心医院妇产科,内蒙古鄂尔多斯017000 [3]山东省临沂市人民医院总院妇一科,山东临沂276000

出　　处：《哈尔滨医科大学学报》2010年第5期420-423,共4页Journal of Harbin Medical University

基　　金：黑龙江省科技攻关计划资金项目(GB06C40106)

摘　　要：目的研究Fas/FasL在绒毛膜癌细胞中的表达,及绒癌细胞与Jurkat细胞共培养诱导T细胞凋亡的机制。方法用免疫荧光染色法检测绒癌细胞系Fas/FasL的阳性表达情况,MTT法检测随时间变化绒癌细胞对Jur-kat细胞的抑制情况,流式细胞仪检测Jurkat细胞与绒癌细胞共培养24 h、中和抗体NOK-2封闭FasL24 h Jurkat细胞的凋亡情况,透射电镜观察细胞的凋亡形态。结果绒癌细胞上均有Fas及FasL的表达,在绒癌细胞上Fas的阳性表达较低,而FasL表达较多(P<0.05)。绒癌细胞与Jurkat细胞共培养可诱导Jurkat细胞凋亡,绒癌细胞与Jurkat细胞共培养后随着时间的延长Jurkat细胞的凋亡率增加(P<0.05),出现特征性凋亡形态特征。用中和抗体NOK-2封闭细胞表面FasL后,Jurkat细胞的凋亡率下降。结论两种绒癌细胞上均有Fas及Fasl的表达,绒癌细胞能抵抗Fas介导的凋亡,其表面的FasL可抑制T细胞的免疫功能,Fas/FasL途径是绒癌细胞免疫逃逸的重要机制之一。Objective To observe the expression of Fas/FasL in choriocarcinoma cell lines（JEG-3 and BeWo） and mechanism of T cell apoptosis induced by co-cultured choriocarcinoma and Jurkat cells.Methods Positive expression of Fas/FasL was detected by immunofluorescence microscopy.The situation of Jurkat cells that co-cultured with choriocarcinoma cells at different time was detected by MTT.The apoptosis of Jurkat cells was detected by flow cytometry when FasL of the choriocarcinoma cell was closed by neutralizing antibody NOK-2 for 24 h.The apoptosis cellular morphology was observed by transmission electronic microscope（TEM）.Results Fas and FasL were expressed in both human choriocarcinoma cell lines.Compared with Jurkat cells,the expression of Fas mRNA was lower,while FasL mRNA was higher in choriocarcinoma cell lines with a significant difference（P0.05）.Co-cultured with Jurkat cells,choriocarcinoma cells induced Jurkat cell apoptosis（P0.05）,with extended culture time,the apoptosis rate of Jurkat cells was notably increased（P0.05）.The apoptosis rate of Jurkat cell was decreased,after FasL of the choriocarcinoma cell was closed by neutralizing antibody NOK-2.Conclu-sion Fas and FasL are both expressed in human choriocarcinoma cell lines.The choriocarcinoma cell can resist the apoptosis induced by Fas/FasL system,the higher expression of FasL can inhibit immunologic function,and its lower Fas expression in T cells can evade immunologic destruction.

关 键 词：人绒癌细胞 JURKAT细胞 FAS/FASL 细胞凋亡 

分 类 号：R737.33[医药卫生—肿瘤]